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Description:
DNA-3-methyladenine glycosylase (EC 3.2.2.21) (3-methyladenine DNAglycosidase) (ADPG) (3-alkyladenine DNA glycosylase) (N-methylpurine-DNA glycosirase).
Molecular weight: 36488
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 2.
07-FEB-2006, entry version 44.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Glires Rodentia Sciurognathi Muroidea Muridae Murinae Mus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein 3MG_MOUSE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X74509 | CAA52615.1 | - |
| EMBL | X75038 | CAA52615.1 | JOINED |
| EMBL | X75039 | CAA52615.1 | JOINED |
| EMBL | X75040 | CAA52615.1 | JOINED |
| EMBL | U10420 | AAA19487.1 | - |
| EMBL | S81162 | NOT_ANNOTATED_CDS | |
| EMBL | S81120 | NOT_ANNOTATED_CDS | |
| EMBL | S81133 | NOT_ANNOTATED_CDS | |
| EMBL | S81134 | NOT_ANNOTATED_CDS | |
| EMBL | BC014754 | AAH14754.1 | - |
| EMBL | M99625 | AAA02577.1 | - |
| PIR | A49003 | A49003. | |
| PIR | I62129 | I62129. | |
| HSSP | P29372 | 1EWN | |
| SMR | Q04841 | 102-315.1 | |
| Ensembl | ENSMUSG00000020287 | Mus musculus.1 | |
| MGI | MGI:97073 | Mpg.1 | |
| GO | GO:0003905 | F:alkylbase DNA N-glycosylase activity | IMP. |
| GO | GO:0006284 | P:base-excision repair | IMP. |
| InterPro | IPR003180 | PurDNA_glycsylse. | |
| PANTHER | PTHR10429 | PurDNA_glycsylse.1 | 1. |
| Pfam | PF02245 | Pur_DNA_glyco | 1. |
| ProDom | PD009649 | PurDNA_glycsylse | 1. |
| TIGRFAMs | TIGR00567 | 3mg | 1. |
Keywords:
DNA damage; DNA repair; Hydrolase; Nuclear protein; Phosphorylation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FEB/N; TISSUE=Spleen;
RX MEDLINE=95134354; PubMed=7832991;
RA Tatsuka M., Ibeanu G.C., Izumi T., Narayan S., Ramana C.V., Kim N.K.,
RA Kang W., Roy G., Mitra S.;
RT "Structural organization of the mouse DNA repair gene, N-methylpurine-
RT DNA glycosylase.";
RL DNA Cell Biol. 14:37-45(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=93169718; PubMed=8435858;
RA Engelward B.P., Boosalis M.S., Chen B.J., Deng Z., Siciliano M.J.,
RA Samson L.D.;
RT "Cloning and characterization of a mouse 3-methyladenine/7-methyl-
RT guanine/3-methylguanine DNA glycosylase cDNA whose gene maps to
RT chromosome 11.";
RL Carcinogenesis 14:175-181(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=96014285; PubMed=8589517;
RA Kielman M.F., Smits R., Bernini L.F.;
RT "Structure of the mouse 3-methyladenine DNA glycosylase gene and exact
RT localization upstream of the alpha-globin gene cluster on chromosome
RT 11.";
RL Mamm. Genome 6:499-504(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 250-324.
RX MEDLINE=93305988; PubMed=8318735;
RA Kielman M., Smits R., Devi T., Fodde R., Bernini L.F.;
RT "Homology of a 130-kb region enclosing the alpha-globin gene cluster,
RT the alpha-locus controlling region, and two non-globin genes in human
RT and mouse.";
RL Mamm. Genome 4:314-323(1993).
Feature:
CHAIN 1 333 DNA-3-methyladenine glycosylase.
/FTId=PRO_0000100066.
MOD_RES 272 272 Phosphoserine (By similarity).
CONFLICT 14 14 S -> A (in Ref. 3).
CONFLICT 319 324 PQQTAC -> LANSLL (in Ref. 5).
Comments:
-!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to
excise 3-methyladenine, and 7-methylguanine from the damaged DNA
polymer formed by alkylation lesions.
-!- CATALYTIC ACTIVITY: Hydrolysis of alkylated DNA, releasing 3-
methyladenine, 3-methylguanine, 7-methylguanine and 7-
methyladenine.
-!- SUBUNIT: Binds MBD1 (By similarity).
-!- SUBCELLULAR LOCATION: Nucleus (Potential).
-!- SIMILARITY: Belongs to the DNA glycosylase MPG family.
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Sequence length: 333
MPARGGSARP GRGSLKPVSV TLLPDTEQPP FLGRARRPGN ARAGSLVTGY HEVGQMPAPL
SRKIGQKKQR LADSEQQQTP KERLLSTPGL RRSIYFSSPE DHSGRLGPEF FDQPAVTLAR
AFLGQVLVRR LADGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYL
IYGMYFCLNV SSQGAGACVL LRALEPLEGL ETMRQLRNSL RKSTVGRSLK DRELCSGPSK
LCQALAIDKS FDQRDLAQDD AVWLEHGPLE SSSPAVVVAA ARIGIGHAGE WTQKPLRFYV
QGSPWVSVVD RVAEQMDQPQ QTACSEGLLI VQK