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Description:
DNA ligase 3 (EC 6.5.1.1) (DNA ligase III) (Polydeoxyribonucleotidesynthase [ATP] 3).
Molecular weight: 1130
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
01-MAY-1997, sequence version 1.
07-MAR-2006, entry version 53.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Glires Rodentia Sciurognathi Muroidea Muridae Murinae Mus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNL3_MOUSE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U66058 | AAC53004.1 | - |
| EMBL | U66057 | AAC53003.1 | - |
| HSSP | P49916 | 1IMO | |
| SMR | P97386 | 89-205.1 | |
| Ensembl | ENSMUSG00000020697 | Mus musculus.1 | |
| MGI | MGI:109152 | Lig3.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| InterPro | IPR012308 | DNA_ligase_A_N. | |
| InterPro | IPR001510 | Znf_PARP. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF04679 | DNA_ligase_A_C | 1. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| Pfam | PF04675 | DNA_ligase_A_N | 1. |
| Pfam | PF00645 | zf-PARP | 1. |
| ProDom | PD004675 | Znf_PolyADPpol | 1. |
| SMART | SM00292 | BRCT | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS50172 | BRCT | 1. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | 1. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
| PROSITE | PS00347 | PARP_ZN_FINGER_1 | 1. |
| PROSITE | PS50064 | PARP_ZN_FINGER_2 | 1. |
Keywords:
Alternative splicing; ATP-binding; Cell cycle; Cell division; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Metal-binding; Nuclear protein; Nucleotide-binding; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
RC TISSUE=Testis;
RX MEDLINE=97154534; PubMed=9001252;
RA Mackey Z.B., Ramos W., Levin D.S., Walter C.A., McCarrey J.R.,
RA Tomkinson A.E.;
RT "An alternative splicing event which occurs in mouse pachytene
RT spermatocytes generates a form of DNA ligase III with distinct
RT biochemical properties that may function in meiotic recombination.";
RL Mol. Cell. Biol. 17:989-998(1997).
Feature:
CHAIN 1 1015 DNA ligase 3.
/FTId=PRO_0000059575.
DOMAIN 939 1015 BRCT.
ZN_FING 94 186 PARP-type.
ACT_SITE 512 512 N6-AMP-lysine intermediate (By
similarity).
VARSPLIC 939 1015 VLLDVFTGVRLYLPPSTPDFKRLKRYFVAFDGDLVQEFDMG
SATHVLGNREKNTDAQLVSSEWIWACIRKRRLIAPC -> R
RRASRQRGRKAMQTGRR (in isoform Beta).
/FTId=VSP_001303.
Comments:
-!- FUNCTION: The alpha isoform interacts with DNA-repair protein
XRCC1 and can correct defective DNA strand-break repair and sister
chromatid exchange following treatment with ionizing radiation and
alkylating agents. The beta isoform does not interact with XRCC1
and may be specifically involved in the completion of homologous
recombination events that occur during meiotic prophase.
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- SUBCELLULAR LOCATION: Nucleus.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=Alpha;
IsoId=P97386-1; Sequence=Displayed;
Name=Beta;
IsoId=P97386-2; Sequence=VSP_001303;
-!- TISSUE SPECIFICITY: The alpha isoform is expressed in all tissues,
while the beta isoform is expressed only in the testis.
-!- DEVELOPMENTAL STAGE: During male germ cell differentiation,
expression of the beta isoform begins in the later stages of
meiotic prophase and ends in the round spermatid stage.
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
-!- SIMILARITY: Contains 1 PARP-type zinc finger.
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Sequence length: 1015
MTLAFKILFP RNLCALGRKE LCLFPEQNRW AAISQFSQWS ETNLLGGCCL LQRRKPVLAL
QRGHLRPRAT HLTFWPGSHV GLCTGPCAMA EQRFCVDYAK RGTAGCKKCK EKIVKGVCRI
GKVVPNPFSE SGGDMKEWYH IKCMFEKLER ARATTKKIED LTELEGWEEL EDNEKEQISQ
HIADLSSKAA ATPKKKAAVQ AKLTTTGQVT SPVKGASFIT STNPRKFSGF SAAKPNNSEQ
APSSPAPGTS LSASKCDPKH KDCLLREFRK LCAMVAENPS YNTKTQIIHD FLQKGSTGST
GDGFHGDVYL TVKLLLPGVI KSVYNLNDKQ IVKLFSRIFN CNPDDMARDL EQGDVSETIR
IFFEQSKSFP PAAKSLLTIQ EVDAFLLHLS KLTKEDEQQQ ALQDIASRCT ANDLKCIRLI
KHDLKMNSGA KHVLDALDPN AYEAFKASRN LQDVVERVLH NEQEVEKDPG RRRALRVQAS
LMTPVQPMLA EACKSIEYAM KKCPNGMFSE IKYDGERVQV HKKGDHFSYF SRSLKPVLPH
KVAHFKDYIP KAFPGGQSMI LDSEVLLIDN NTGKPLPFGT LGVHKKAAFQ DANLCLFVFD
CIYFNDVSLM DRPLCERRKF LHDNMVEIRN RIMFSEMKQV TKASDLADMI NRVIREGLEG
LVLKDVKGTY EPGKRHWLKV KKDYLNEGAM ADTADLVVLG VFLIGQGSKG GMMSIFLMGC
YDPDSQKWCT VTKCAGGHDD ATLARLQKEL VMVKISKDPS KIPSWLKINK IYYPDFIVPD
PKKAAVWEIT GAEFSRSEAH TADGISIRFP RCTRIRDDKD WKSATNLPQL KELYQLSKDK
ADFAVVAGDE ASPTTGGSSG ENEGTAGSAG PCKGPPSKSS ASAKTTEQKL NSPSSRGGIK
PIPKHSPMKP GEKLAVKSSP VKVGMKRKAT DETPCLKKVL LDVFTGVRLY LPPSTPDFKR
LKRYFVAFDG DLVQEFDMGS ATHVLGNREK NTDAQLVSSE WIWACIRKRR LIAPC