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Description:
Flap endonuclease 1 (EC 3.1.-.-).
Molecular weight: 42315
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
01-FEB-1995, sequence version 1.
07-FEB-2006, entry version 41.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Glires Rodentia Sciurognathi Muroidea Muridae Murinae Mus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein FEN1_MOUSE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L26320 | AAC37664.1 | - |
| PIR | A53730 | A53730. | |
| HSSP | O93634 | 1B43 | |
| Ensembl | ENSMUSG00000024742 | Mus musculus.1 | |
| MGI | MGI:102779 | Fen1.1 | |
| GO | GO:0005634 | C:nucleus | TAS. |
| GO | GO:0003677 | F:DNA binding | IDA. |
| GO | GO:0004527 | F:exonuclease activity | IDA. |
| GO | GO:0048256 | F:flap endonuclease activity | IDA. |
| GO | GO:0000287 | F:magnesium ion binding | IDA. |
| GO | GO:0030145 | F:manganese ion binding | IDA. |
| GO | GO:0006281 | P:DNA repair | IMP. |
| GO | GO:0006260 | P:DNA replication | IMP. |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR006086 | XPG_I. | |
| InterPro | IPR006085 | XPG_N. | |
| InterPro | IPR006084 | XPGC_Rad. | |
| Pfam | PF00867 | XPG_I | 1. |
| Pfam | PF00752 | XPG_N | 1. |
| PRINTS | PR00853 | XPGRADSUPER. | |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00484 | XPGI | 1. |
| SMART | SM00485 | XPGN | 1. |
| PROSITE | PS00841 | XPG_1 | 1. |
| PROSITE | PS00842 | XPG_2 | 1. |
Keywords:
Direct protein sequencing; Endonuclease; Hydrolase; Nuclear protein; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 260-275 AND
RP 331-352.
RC STRAIN=BALB/c;
RX MEDLINE=95011546; PubMed=7926735;
RA Harrington J.J., Lieber M.R.;
RT "Functional domains within FEN-1 and RAD2 define a family of
RT structure-specific endonucleases: implications for nucleotide excision
RT repair.";
RL Genes Dev. 8:1344-1355(1994).
Feature:
CHAIN 1 378 Flap endonuclease 1.
/FTId=PRO_0000154070.
REGION 1 102 N-domain.
REGION 120 251 I-domain.
Comments:
-!- FUNCTION: Endonuclease that cleave the 5'overhanging flap
structure that is generated by displacement synthesis when DNA
polymerase encounters the 5'end of a downstream Okazaki fragment.
It fails to cleave other DNA structures, including 3'flaps and
single stranded DNA.
-!- SUBUNIT: Interacts with PCNA (By similarity).
-!- SUBCELLULAR LOCATION: Nucleus (Probable).
-!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
subfamily.
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Sequence length: 378
MGIHGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET
TSLMGMFYRT IRMENGIKPV YVFDGKPPQL KSGELAKRSE RRAEAEKQLQ QAQEAGMEEE
VEKFTKRLVK VTKQHNDECK HLLSLMGIPY LDAPSEAEAS CAALAKAGKV YAAATEDMDC
LTFGSPVLMR HLTASEAKKL PIQEFHLSRV LQELGLNQEQ FVDLCILLGS DYCESIRGIG
AKRAVDLIQK HKSIEEIVRR LDPSKYPVPE NWLHKEAQQL FLEPEVVDPE SVELKWSEPN
EEELVKFMCG EKQFSEERIR SGVKRLSKSR QGSTQGRLDD FFKVTGSLSS AKRKEPEPKG
PAKKKAKTGG AGKFRRGK