Description:
N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase(EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18)(AP lyase)].
Molecular weight: 38883
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
01-DEC-2000, sequence version 2.
07-FEB-2006, entry version 52.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Glires Rodentia Sciurognathi Muroidea Muridae Murinae Mus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGG1_MOUSE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF003596 | AAB61289.1 | - |
| EMBL | U88621 | AAB68616.1 | - |
| EMBL | AF000669 | AAB63151.1 | - |
| EMBL | AF012916 | AAB94512.1 | - |
| EMBL | AF012912 | AAB94512.1 | JOINED |
| EMBL | AF012913 | AAB94512.1 | JOINED |
| EMBL | AF012914 | AAB94512.1 | JOINED |
| EMBL | AF012915 | AAB94512.1 | JOINED |
| EMBL | U96711 | AAB81133.1 | - |
| EMBL | Y11247 | CAA72117.1 | - |
| EMBL | Y13479 | CAA73883.1 | - |
| EMBL | AJ001307 | CAB65240.1 | - |
| PIR | T46962 | T46962. | |
| HSSP | Q9UIK1 | 1LWY | |
| SMR | O08760 | 13-323.1 | |
| Ensembl | ENSMUSG00000030271 | Mus musculus.1 | |
| MGI | MGI:1097693 | Ogg1.1 | |
| GO | GO:0005739 | C:mitochondrion | IMP. |
| GO | GO:0005634 | C:nucleus | IMP. |
| GO | GO:0008534 | F:purine-specific oxidized base lesion DNA N-... | IDA. |
| GO | GO:0006284 | P:base-excision repair | IDA. |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR004577 | Ogg. | |
| InterPro | IPR012904 | OGG_N. | |
| InterPro | IPR012294 | TFIID_C/glycos_N. | |
| Pfam | PF00633 | HHH | 1. |
| Pfam | PF00730 | HhH-GPD | 1. |
| Pfam | PF07934 | OGG_N | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| TIGRFAMs | TIGR00588 | ogg | 1. |
Keywords:
DNA damage; DNA repair; Glycosidase; Hydrolase; Lyase; Multifunctional enzyme; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=97352815; PubMed=9207108; DOI=10.1073/pnas.94.14.7429;
RA Rosenquist T.A., Zharkov D.O., Grollman A.P.;
RT "Cloning and characterization of a mammalian 8-oxoguanine DNA
RT glycosylase.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:7429-7434(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=97342862; PubMed=9197244; DOI=10.1016/S0960-9822(06)00187-4;
RA Lu R., Nash H.M., Verdine G.L.;
RT "A mammalian DNA repair enzyme that excises oxidatively damaged
RT guanines maps to a locus frequently lost in lung cancer.";
RL Curr. Biol. 7:397-407(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=97330655; PubMed=9187114;
RA Aburatani H., Hippo Y., Ishida T., Takashima R., Matsuba C.,
RA Kodama T., Takao M., Yasui A., Yamamoto K., Asano M., Fukasawa K.,
RA Yoshinari T., Inoue H., Otsuka E., Nishimura S.;
RT "Cloning and characterization of mammalian 8-hydroxyguanine-specific
RT DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM
RT homologue.";
RL Cancer Res. 57:2151-2156(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C3H/HeN;
RX MEDLINE=98096582; PubMed=9434942; DOI=10.1007/s003359900675;
RA Tani M., Shinmura K., Kohno T., Takenoshita S., Nagamachi Y.,
RA Yokota J.;
RT "Genomic structure and chromosomal localization of the mouse Ogg1 gene
RT that is involved in the repair of 8-hydroxyguanine in DNA damage.";
RL Mamm. Genome 9:32-37(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Lung;
RX MEDLINE=98026907; PubMed=9321410; DOI=10.1093/emboj/16.20.6314;
RA Bjoras M., Luna L., Johnsen B.E., Hoff E., Haug T., Rognes T.,
RA Seeberg E.;
RT "Opposite base-dependent reactions of a human base excision repair
RT enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites.";
RL EMBO J. 16:6314-6322(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J;
RA Radicella J.P., Reille F., Dherin C., Boiteux S.;
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=129/Sv;
RA Johnsen B., Luna L., Rognes T., Seeberg E.;
RT "Complete genomic DNA sequence of the Mus musculus 8-oxoguanine DNA
RT glycosylase 1 gene (OGH1).";
RL Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 345 N-glycosylase/DNA lyase.
/FTId=PRO_0000058592.
ACT_SITE 249 249 Schiff-base intermediate with DNA (By
similarity).
BINDING 149 149 DNA (By similarity).
BINDING 154 154 DNA (By similarity).
BINDING 204 204 DNA (By similarity).
BINDING 266 266 8-oxoguanine; via carbonyl oxygen (By
similarity).
BINDING 268 268 8-oxoguanine (By similarity).
BINDING 270 270 DNA (By similarity).
BINDING 287 287 DNA (By similarity).
BINDING 315 315 8-oxoguanine (By similarity).
BINDING 319 319 8-oxoguanine (By similarity).
CONFLICT 10 10 S -> Q (in Ref. 2).
CONFLICT 23 25 WAS -> SVA (in Ref. 2).
CONFLICT 239 239 A -> G (in Ref. 2).
CONFLICT 299 299 L -> S (in Ref. 6).
CONFLICT 329 329 S -> F (in Ref. 1).
CONFLICT 336 336 R -> H (in Ref. 5 and 7).
Comments:
-!- FUNCTION: DNA repair enzyme that incises DNA at 8-oxoG residues.
Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-
methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-
lyase activity that nicks DNA 3' to the lesion.
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage near apurinic or
apyrimidinic sites to products with 5'-phosphate.
-!- SUBCELLULAR LOCATION: Nucleus (By similarity).
-!- TISSUE SPECIFICITY: Highest expression in testis.
-!- SIMILARITY: Belongs to the type-1 OGG1 family.
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Sequence length: 345
MLFRSWLPSS MRHRTLSSSP ALWASIPCPR SELRLDLVLA SGQSFRWKEQ SPAHWSGVLA
DQVWTLTQTE DQLYCTVYRG DDSQVSRPTL EELETLHKYF QLDVSLAQLY SHWASVDSHF
QRVAQKFQGV RLLRQDPTEC LFSFICSSNN NIARITGMVE RLCQAFGPRL IQLDDVTYHG
FPNLHALAGP EAETHLRKLG LGYRARYVRA SAKAILEEQG GPAWLQQLRV APYEEAHKAL
CTLPGVGAKV ADCICLMALD KPQAVPVDVH VWQIAHRDYG WHPKTSQAKG PSPLANKELG
NFFRNLWGPY AGWAQAVLFS ADLRQPSLSR EPPAKRKKGS KRPEG