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Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 17741
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
01-JUN-1998, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Bacteria Cyanobacteria Nostocales Nostocaceae Nostoc.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_ANASP:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF046871 | AAC03106.1 | - |
| EMBL | BA000019 | BAB73996.1 | - |
| PIR | AB2093 | AB2093. | |
| HSSP | P24239 | 1HJR | |
| GenomeReviews | BA000019_GR | all2297.1 | |
| BioCyc | NOST-PCC-01:NOST-PCC-01-002292-MONOMER | -.1 | |
| BioCyc | NSP103690:ALL2297-MONOMER | -.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| TIGRFAMs | TIGR00228 | ruvC | 1. |
| PROSITE | PS01321 | RUVC | 1. |
Keywords:
Complete proteome; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=99011350; PubMed=9794762; DOI=10.1126/science.282.5390.935;
RA Yoon H.-S., Golden J.W.;
RT "Heterocyst pattern formation controlled by a diffusible peptide.";
RL Science 282:935-938(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=21595285; PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S.,
RA Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T.,
RA Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA Nakazaki N., Shimpo S., Sugimoto M., Takazawa M., Yamada M.,
RA Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
Feature:
CHAIN 1 163 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183075.
METAL 9 9 Magnesium (By similarity).
METAL 76 76 Magnesium (By similarity).
METAL 148 148 Magnesium (By similarity).
METAL 151 151 Magnesium (By similarity).
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group
(By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
-!- SIMILARITY: Belongs to the ruvC family.
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Sequence length: 163
MEKRILGLDP GLAILGFGAI TCTPGLTQLQ STKVNVLDFG VIKTSADIEI GQRLCTLFDD
LHTVIDQLQP DVVAIEKLFF YRMSSTIVVA QARGVVMLAL AQHHLPYVEF TPAQIKLALT
GYGNADKSEV QEAVARELDL AEIPRPDDAA DALAVALTAW YQM