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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 75587
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
01-MAR-2002, sequence version 1.
07-MAR-2006, entry version 23.
Phylogenetic order:
Bacteria Cyanobacteria Nostocales Nostocaceae Nostoc.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_ANASP:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000019 | BAB73089.1 | - |
| PIR | AI1947 | AI1947. | |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | BA000019_GR | all1132.1 | |
| BioCyc | NOST-PCC-01:NOST-PCC-01-001130-MONOMER | -.1 | |
| BioCyc | NSP103690:ALL1132-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=21595285; PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S.,
RA Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T.,
RA Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA Nakazaki N., Shimpo S., Sugimoto M., Takazawa M., Yamada M.,
RA Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
Feature:
CHAIN 1 665 UvrABC system protein B.
/FTId=PRO_0000138377.
DOMAIN 626 661 UVR.
NP_BIND 38 45 ATP (Potential).
MOTIF 91 114 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 665
MTEFGLQAPF SPTGDQPSAI AQLVASIEGG NRYQTLLGAT GTGKTFSIAA VIEKIGRPTL
VLAHNKTLAA QLCNELREFF PNNAVEYFVS YYDYYQPEAY IPVTDTYIEK TAAINDEIDM
LRHSATRSLF ERRDVIVVAS ISCIYGLGIP AEYLKAAIPL QIGMEVNQRE ILRDLASVQY
SRNDVEMGRG RFRVRGDVLE IGPAYEDRII RVEFFGDEID AIRYIDPVTG EIINSLQAVN
IYPARHFVTP EERLEVACED IAYELKQRKA ELEETGKLVE AQRIDQRTRY DLEMLREVGY
CNGVENYSRH LAGRQAGEPP ESLIDYFPKD WLLVIDESHV TVPQIRGMYN GDQARKKVLI
EHGFRLPSAA DNRPLKAEEF WQKVNQCIFV SATPGNWELE ISENRIVEQV IRPTGVIDPE
ISVRPTEGQI DDLLGEIKDR IDLHERVLIT TLTKRMAEDL TEYLQEHGVK VRYLHSEINS
IQRIEILQDL RQGSFDVLVG VNLLREGLDL PEVSLVAIMD ADKEGFLRAE RSLIQTIGRA
ARHIRGQAIL YADNMTDSMI KAIEETDRRR GIQVAYNKLH GITPQPIVKK SSNAILSFLE
VSRRLNATDL KVVEEHIDEL PLEEIPNLID KLEAQMKEAS KKLEFEEAAK LRDRIKQLRD
KLVGR