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Description:
DNA polymerase I (EC 2.7.7.7) (POL I).
Molecular weight: 11028
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 44.
Phylogenetic order:
Bacteria Cyanobacteria Chroococcales Synechocystis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO1_SYNY3:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000022 | BAA10748.1 | - |
| PIR | S77056 | S77056. | |
| HSSP | P19821 | 1KTQ | |
| GenomeReviews | BA000022_GR | slr0707.1 | |
| BioCyc | SSP1148:SLR0707-MONOMER | -.1 | |
| InterPro | IPR002562 | 3_5_exonuclease. | |
| InterPro | IPR002421 | 5_3_exonuclease. | |
| InterPro | IPR001098 | DNA_pol. | |
| InterPro | IPR002298 | DNA_polI. | |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR012337 | RNaseH_fold. | |
| Pfam | PF01612 | 3_5_exonuc | 1. |
| Pfam | PF01367 | 5_3_exonuc | 1. |
| Pfam | PF02739 | 5_3_exonuc_N | 1. |
| Pfam | PF00476 | DNA_pol_A | 1. |
| PRINTS | PR00868 | DNAPOLI. | |
| SMART | SM00474 | 35EXOc | 1. |
| SMART | SM00475 | 53EXOc | 1. |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00482 | POLAc | 1. |
| TIGRFAMs | TIGR00593 | pola | 1. |
| PROSITE | PS00447 | DNA_POLYMERASE_A | FALSE_NEG. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=96127529; PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb
RT region from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT entire genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
Feature:
CHAIN 1 986 DNA polymerase I.
/FTId=PRO_0000101255.
DOMAIN 1 303 5'-3' exonuclease.
DOMAIN 304 592 3'-5' exonuclease.
REGION 593 986 Polymerase.
Comments:
-!- FUNCTION: In addition to polymerase activity, this DNA polymerase
exhibits 3' to 5' and 5' to 3' exonuclease activity (By
similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBUNIT: Single-chain monomer with multiple functions (By
similarity).
-!- SIMILARITY: Belongs to the DNA polymerase type-A family.
-!- SIMILARITY: Contains 1 3'-5' exonuclease domain.
-!- SIMILARITY: Contains 1 5'-3' exonuclease domain.
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Sequence length: 986
MFMSAKSPLL LLVDGHSLAF RAYYAFGLSK KGPLRTTAGI PTSVCFGFLN SLMQVMESQK
PAAIAIAFDR REPTFRHEAD GAYKSNRQET PEDFAEDLSY LQQLLEALNL QTITYAGYEA
DDILGTLACQ GSDAGYQVKI LSGDRDLFQL VSPEKNISVL YLTRNPFSSN TGYDELDWQG
VVDKMGVTPA QIVDFKALCG DKSDCIPGIN GIGEKTAIKL LAEYETLEKV YENLAQIKGA
LKTRLDNGKD DAMHSQMLAR IVVDVPLPVT WEDLQLTGFS TDRLVPLLEK LELRTFIDKI
QAFHRNFSDN QSPVPMGNEA DNGEPKKTVK AKKSKEKVNP DDSQQLSLFD GVPVVNQEDG
LITIQLPKQI QPQIITTIAQ LEALVEELKK HTDADFPVAW DTETDSLDPL VANLVGIGCA
WGQEPNQVAY IPLKHHQGEQ LSLGIIKDLL GEILGNAIYP KVLQNAKFDR RVLAHHGIEL
GGVVLDTMLA SYVLQPEETH NLTDLCRRYN LGLVALSYKD LGLKKDQTIA DLPLETAGQY
CGLDCYATYL LASKLQKELD QYPELKEILK EIEQPLEKIL AAMEDRGIRI DCDYLQTLSQ
QLAENLLTIE TAAYEAAGES FNLSSPKQLG TILFDKLGLD RKKSRKTKTG YSTDHATLEK
LQGDHPIIDA ILEHRTLAKL KSTYVDALPE LVNGQTQRIH TDFNQAVTST GRLSSSNPNL
QNIPIRSDFS RQIRRAFLPQ KDWLLVSADY SQIELRILAH LSQEPVLLQA YGDRQDVHGV
TAKLLFGKED ITPAERNLGK TINFGVIYGM GAQRFARETG ISAVEGREFI DRYHRTYAQV
FDYLETMKLE AIAKGYVTTI VGRRRYFNFV TEALRQLRGK TVTELDLVDV KMNYNDAQLL
RSAANAPIQG SSADIIKIAM VKLAKLLESY QTRMLLQVHD ELIFEMPPEE WEELAPLIQN
TMEQALTLSV PLVVEMHRGS NWMEAK