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Description:
Endonuclease III (EC 4.2.99.18) (DNA-(apurinic or apyrimidinic site)lyase).
Molecular weight: 24377
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
01-FEB-1997, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Bacteria Cyanobacteria Chroococcales Synechocystis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END3_SYNY3:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000022 | BAA17762.1 | - |
| PIR | S77204 | S77204. | |
| HSSP | P20625 | 2ABK | |
| GenomeReviews | BA000022_GR | slr1822.1 | |
| BioCyc | SSP1148:SLR1822-MONOMER | -.1 | |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR004035 | EndoIII_FCL. | |
| InterPro | IPR004036 | EndoIII_HhH. | |
| InterPro | IPR003651 | FeS_bind. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR005759 | Nth. | |
| Pfam | PF00633 | HHH | 1. |
| Pfam | PF00730 | HhH-GPD | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| SMART | SM00525 | FES | 1. |
| TIGRFAMs | TIGR01083 | nth | 1. |
| PROSITE | PS00764 | ENDONUCLEASE_III_1 | 1. |
| PROSITE | PS01155 | ENDONUCLEASE_III_2 | 1. |
Keywords:
4Fe-4S; Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase; Iron; Iron-sulfur; Lyase; Metal-binding; Multifunctional enzyme.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT entire genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
Feature:
CHAIN 1 219 Endonuclease III.
/FTId=PRO_0000102223.
METAL 197 197 Iron-sulfur (4Fe-4S) (By similarity).
METAL 204 204 Iron-sulfur (4Fe-4S) (By similarity).
METAL 207 207 Iron-sulfur (4Fe-4S) (By similarity).
METAL 213 213 Iron-sulfur (4Fe-4S) (By similarity).
Comments:
-!- FUNCTION: Has both an apurinic and/or apyrimidinic endonuclease
activity and a DNA N-glycosylase activity. Incises damaged DNA at
cytosines, thymines and guanines. Acts on a damaged strand, 5'
from the damaged site (By similarity).
-!- CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or
apyrimidinic site in DNA is broken by a beta-elimination reaction,
leaving a 3'-terminal unsaturated sugar and a product with a
terminal 5'-phosphate.
-!- COFACTOR: Binds 1 4Fe-4S cluster which is not important for the
catalytic activity, but which is probably involved in the proper
positioning of the enzyme along the DNA strand (By similarity).
-!- SIMILARITY: Belongs to the nth/mutY family.
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Sequence length: 219
MSSLLRKMAS KKQRATEILL ILKKLYPGAT CSLDYQTPVQ LLVATILSAQ CTDERVNKVT
PALFQRYPDA NALAYGDRQE IEELIHSTGF FRNKAKNIQG ACRKIVEEFD GEVPQRMEEL
LTLPGVARKT ANVVLAHAFG ILAGVTVDTH VKRLSQRLGL TKATDPIRIE RDLMKLIPQP
DWENFSIHII YHGRAVCAAR KPLCGECQLA HLCPSAQAS