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Description:
Circadian clock protein kinase kaiC (EC 2.7.1.37).
Molecular weight: 58294
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
01-FEB-1997, sequence version 1.
07-MAR-2006, entry version 31.
Phylogenetic order:
Bacteria Cyanobacteria Chroococcales Synechocystis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein KAIC_SYNY3:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000022 | BAA18762.1 | - |
| PIR | S76850 | S76850. | |
| SMR | P74646 | 17-498.1 | |
| GenomeReviews | BA000022_GR | slr0758.1 | |
| BioCyc | SSP1148:SLR0758-MONOMER | -.1 | |
| HAMAP | MF_01836 | - | 1. |
| InterPro | IPR010624 | KaiC. | |
| InterPro | IPR004504 | RadA. | |
| Pfam | PF06745 | KaiC | 2. |
| PRINTS | PR01874 | DNAREPAIRADA. | |
| PROSITE | PS51146 | KAIC | 2. |
Keywords:
ATP-binding; Biological rhythms; Complete proteome; DNA-binding; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphorylation; Repeat; Repressor; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT entire genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
Feature:
CHAIN 1 519 Circadian clock protein kinase kaiC.
/FTId=PRO_0000217786.
DOMAIN 20 261 KaiC 1.
DOMAIN 262 494 KaiC 2.
NP_BIND 47 54 ATP (By similarity).
NP_BIND 289 296 ATP (By similarity).
METAL 296 296 Magnesium (By similarity).
METAL 319 319 Magnesium (By similarity).
METAL 320 320 Magnesium (By similarity).
METAL 379 379 Magnesium (By similarity).
MOD_RES 433 433 Phosphothreonine (by autocatalysis) (By
similarity).
Comments:
-!- FUNCTION: Core component of the kaiABC clock protein complex,
which constitutes the main circadian regulator in cyanobacteria.
Binds to DNA. The kaiABC complex may act as a promoter-nonspecific
transcription regulator that represses transcription, possibly by
acting on the state of chromosome compaction (By similarity).
-!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
-!- COFACTOR: Magnesium (By similarity).
-!- ENZYME REGULATION: The interaction with kaiA enhances its
phosphorylation status, while the interaction with kaiB decreases
it. A kaiA dimer is sufficient to enhance kaiC phosphorylation (By
similarity).
-!- SUBUNIT: Homohexamer; hexamerization is dependent on ATP-binding.
Core component of the kaiABC complex, at least composed of a kaiC
homohexamer, a kaiB dimer and two kaiA dimers. Interacts directly
with sasA (By similarity).
-!- DOMAIN: The kaiC domains mediate the interaction with kaiA (By
similarity).
-!- PTM: Phosphorylated on serine/threonine residues by autocatalysis.
Both phosphorylated and unphosphorylated forms exist. Can probably
autophosphorylate and autodephosphorylate. Phosphorylated form
correlates with clock speed (By similarity).
-!- SIMILARITY: Belongs to the kaiC family.
-!- SIMILARITY: Contains 2 kaiC domains.
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Sequence length: 519
MNLPIVNERN RPDVPRKGVQ KIRTVIEGFD EITHGGLPIG RTTLVSGTSG TGKTLLAVQF
LYQGIHHFDY PGLFITFEES PSDIIENAYS FGWDLQQLID DGKLFILDAS PDPEGQEVVG
TFDLSALIER IQYAVRKYKA KLVSIDSVTA VFQQYDAASV VRREIFRLVA RLKQLQVTSI
MTTERVEEYG PIARFGVEEF VSDNVVVLRN VLEGERRRRT VEILKLRGTT HMKGEYPFTI
THDGINIFPL GAMRLTQRSS NARISSGVQT LDEMCGGGFF KDSIILATGA TGTGKTLLVS
KFLQEGCRQR ERAILFAYEE SRAQLSRNAS SWGIDFEEME HKGLLKLLCT YPESAGLEDH
LQMIKSEISE FKPSRIAIDS LSALARGVTN NAFRQFVIGV TGYAKQEEIT GFFTNTTDQF
MGAHSITESH ISTITDTILM LQYVEIRGEM SRALNVFKMR GSWHDKGIRE YSISHDGPDI
RDSFRNYERI ISGSPTRISV DEKSELSRIV RGVKDKTAE