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Description:
Transcription-repair coupling factor (EC 3.6.1.-) (TRCF) (ATP-dependent helicase mfd).
Molecular weight: 1345
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 38.
Phylogenetic order:
Bacteria Cyanobacteria Chroococcales Synechocystis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MFD_SYNY3:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000022 | BAA10395.1 | - |
| PIR | S76549 | S76549. | |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | BA000022_GR | sll0377.1 | |
| BioCyc | SSP1148:SLL0377-MONOMER | -.1 | |
| InterPro | IPR003711 | CarD_TRCF. | |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR004576 | Mfd. | |
| InterPro | IPR005118 | TRCF. | |
| Pfam | PF02559 | CarD_TRCF | 1. |
| Pfam | PF00270 | DEAD | 1. |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF03461 | TRCF | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00580 | mfd | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=96127529; PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb
RT region from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT entire genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
Feature:
CHAIN 1 1199 Transcription-repair coupling factor.
/FTId=PRO_0000102174.
NP_BIND 673 680 ATP (Potential).
MOTIF 775 778 DEEQ box.
Comments:
-!- FUNCTION: Necessary for strand-specific repair. A lesion in the
template strand blocks the RNA polymerase complex (RNAP). The
RNAP-DNA-RNA complex is specifically recognized by TRCF which
releases RNAP and the truncated transcript; the TCRF may replace
RNAP at the lesion site and then recruit the uvrA/B/C repair
system (By similarity).
-!- SIMILARITY: In the N-terminal section; belongs to the uvrB family.
-!- SIMILARITY: In the C-terminal section; belongs to the helicase
family. RecG subfamily.
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Sequence length: 1199
MAEPRAVLKQ GNRNRDKDSV GLGNRLRFVS ISSALNPMTF SSILRHLQTL PLSKDLREKL
QKRGHVQLSG LPRLPKGLIV SSLAQSLEKN LLVITATLEE AGRWTAQLEL MGWQTVNFYP
TSEASPYDPG RLESEMVWGQ MQVLAELIQG HQVKGKAIVA TEKALQPHLP PVATLREYCL
ALRRGQEMDS KSLELTLARL GYERGSTVET EGQWSRRGDI VDIFPVSAEL PVRLEWFGDE
LEKIREFDPA SQRSLDDLTG LVLTPTSFDQ VIEPALNAQA IDLSAWGEDA ETEQLFGKEG
LQRFLGLAFT EPACLLDYLP TETVCVLDEP EQCAAHSERW FEAVAQDWSA LQLPNLPQLH
RNFSALGDRL KTDFQYITLS EIHEANGDSL DISARPIPTM PHQFAKLAEI LRGKREIYSG
LTLGQYSTWL ISAQPSRTVS LLQEHDCAVQ FIANPRDYPA IEKSQIQRTA VTLKYSGLAE
LEGFILPTFR LVLVTDRELF GQHALATPEY VRKRRRATSK QVDINKLSPG DYVVHKSHGI
GKFLKLDALA NREYLMIQYA DGILRVPADS LDSLSRFRHT GTRPPELHKM GGKVWEATKN
KVRKAVKKLA VDLLNLYAKR AKQVGYAYPP DSPWQQELED SFPYQPTPDQ LKAVQDVKRD
LEGDRPMDRL VCGDVGFGKT EVAVRAIFKA VTSGNKQVAL LAPTTVLTQQ HYHTLKERFA
PYPITIGLLN RFRTASEKKE ILAKLKSGEL DIVVGTQQVL GTSVKFKDLG LLVIDEEQRF
GVNQKEKIKT LKTEVDVLTL TATPIPRTLY MSLSGIREMS LITTPPPSRR PIKTHLSPYN
PEVIRTAIRN ELDRGGQVFY VVPRIEGIEE LGGQLRQMVP SARIAIGHGQ MEESELESTM
LAFNDGEADI LVCTTIIEAG LDIPRVNTII VEDAQKFGLA QLYQLRGRVG RSGIQAHAWL
LYPNQKQLTE KARLRLRALQ EFSQLGSGYQ LATRDMEIRG VGNLLGAEQS GQMEAIGYEF
YMEMLQDAIK EIQGQEIPKV EDTQIDLPLT AFIPSDYIPD LEEKMAAYRR ITSIESTDEL
PKIALDWGDR YGMLPSPVEE LFKVVKLKHL AKSLGFSRIK VEGKQNLVLE TPMEEPAWKL
LAENLPNHLQ SRFVYSAKKV VVRGLGVLAP AKQMDNLIDW FGKMQGALPE TKMETLVGK