|
|
|
|
|
|
Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 18193
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Bacteria Cyanobacteria Chroococcales Synechocystis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_SYNY3:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000022 | BAA10861.1 | - |
| PIR | S76014 | S76014. | |
| GenomeReviews | BA000022_GR | sll0896.1 | |
| BioCyc | SSP1148:SLL0896-MONOMER | -.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| PROSITE | PS01321 | RUVC | 1. |
Keywords:
Complete proteome; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=96127529; PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb
RT region from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT entire genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
Feature:
CHAIN 1 167 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183138.
METAL 14 14 Magnesium (By similarity).
METAL 75 75 Magnesium (By similarity).
METAL 147 147 Magnesium (By similarity).
METAL 150 150 Magnesium (By similarity).
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group
(By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
-!- SIMILARITY: Belongs to the ruvC family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 167
MDQTEAAQRW LGIDPGLAII GWAILDGQPD LSAHLVDYGI IETSKNLSTP ERLVEIEQDI
HVLLEEFSPQ AIAMEMPFFS RQIKAAGGVM QALGVINLVI YREKSIQPVF LHQSSWKCHL
GNGKANKAEV AAMVQNFFGL EDLPIDDSVD AIAIALAGLN GVRNDIG