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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 76849
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 40.
Phylogenetic order:
Bacteria Cyanobacteria Chroococcales Synechocystis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_SYNY3:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000022 | BAA10309.1 | - |
| PIR | S74391 | S74391. | |
| HSSP | Q56243 | 1C4O | |
| GenomeReviews | BA000022_GR | sll0459.1 | |
| BioCyc | SSP1148:SLL0459-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=96127529; PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb
RT region from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT entire genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
Feature:
CHAIN 1 669 UvrABC system protein B.
/FTId=PRO_0000138438.
DOMAIN 628 663 UVR.
NP_BIND 40 47 ATP (Potential).
MOTIF 93 116 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 669
MTSDLFSLHA PFRPTGDQPT AIASLIESLQ GEHKFQTLLG ATGTGKTFTM ASVIAELGRP
TLVLAHNKTL AAQLCNELRQ FFPENAVEYF ISYYDYYQPE AYIPVTDTYI EKTASINDEI
DMLRHSATRS LFERRDVIVV ASISCIYGLG IPSQYLKAAV PLQVGAEYDP RLVIRDLVNV
QYSRNDVELQ RGRFRLKGDV LEIVPAYEDR VIRIEFFGDE VEALRLIDPV SGEILQSLDR
ISIYPARHFV TPEETLEKAC QQIQTEMEQQ VAFLEKNNLL VEAQRLSQRT RYDLEMLREV
GYCNGVENYS RYLADRQAGE PPECLVDYFP EDWLLVIDES HVTIPQLRGM YNGDQARKKV
LIDHGFRLPS AADNRPLKAE EFWQKVKQCV FVSATPGVWE IEQSEARVIE QVIRPTGVLD
PEIFVRPTTG QVDDLYGEIQ TRVKLKERVL ITTLTKRMAE DLTDYFSERG IKVQYLHSEI
QSIQRIEILQ ALRDGEFDVL IGVNLLREGL DLPEVSLVAI MDADKEGFLR AERSLIQTIG
RAARHIRGQA ILYADNFTDS MQKAIAETER RRKIQQEYNE KHGITPQPIN KRANNAILQF
LDISRRLNSQ QLEEVYEQAQ DLPLEKIPDL IQQLEEKMQE AAKKQEFEVA AIYRDRIQHL
RDRLLGHKK