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Description:
ATP-dependent DNA helicase pcrA (EC 3.6.1.-).
Molecular weight: 83871
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
28-NOV-2003, sequence version 2.
07-FEB-2006, entry version 31.
Phylogenetic order:
Bacteria Firmicutes Bacillales Staphylococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein PCRA_STAAU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M63176 | AAA72091.1 | ALT_FRAME |
| PIR | S39923 | S39923. | |
| HSSP | P56255 | 1QHG | |
| InterPro | IPR005751 | PcrA. | |
| InterPro | IPR000212 | UvrD-helicase. | |
| PANTHER | PTHR11070 | UvrD-helicase.1 | 1. |
| Pfam | PF00580 | UvrD-helicase | 1. |
| TIGRFAMs | TIGR01073 | pcrA | 1. |
Keywords:
ATP-binding; DNA-binding; Helicase; Hydrolase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANT PCRA3.
RC STRAIN=SA20 / NCTC 8325;
RX MEDLINE=94049679; PubMed=8232203; DOI=10.1007/BF00280216;
RA Iordanescu S.;
RT "Characterization of the Staphylococcus aureus chromosomal gene pcrA,
RT identified by mutations affecting plasmid pT181 replication.";
RL Mol. Gen. Genet. 241:185-192(1993).
Feature:
CHAIN 1 728 ATP-dependent DNA helicase pcrA.
/FTId=PRO_0000102061.
NP_BIND 27 34 ATP (Potential).
MUTAGEN 61 61 T->I: In pcrA3; affects plasmid pT181
replication.
Comments:
-!- FUNCTION: Essential helicase. May act as a helicase in plasmid
pT181 replication.
-!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
-!- CAUTION: Ref.1 sequence differs from that shown due to a
frameshift in position 666.
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Sequence length: 728
MNALLNHMNT EQSEAVKTTE GPLLIMAGAG SGKTRVLTHR IAYLLDEKDV SPYNVLAITF
TNKAAREMKE RVQKLVGDQA EVIWMSTFHS MCVRILRRDA DRIGIERNFT IIDPTDQKSV
IKDVLKNENI DSKKFEPRMF IGAISNLKNE LKTPADAQKE ATDYHSQMVA TVYSGYQRQL
SRNEALDFDD LIMTTINLFE RVPEVLEYYQ NKFQYIHVDE YQDTNKAQYT LVKLLASKFK
NLCVVGDSDQ SIYGWRGADI QNILSFEKDY PEANTIFLEQ NYRSTKTILN AANEVIKNNS
ERKPKGLWTA NTNGEKIHYY EAMTERDEAE FVIREIMKHQ RNGKKYQDMA ILYRTNAQSR
VLEETFMKSN MPYTMVGGQK FYDRKEIKDL LSYLRIIANS NDDISLQRII NVPKRGVGPS
SVEKVQNYAL QNNISMFDAL GEADFIGLSK KVTQECLNFY ELIQSLIKEQ EFLEIHEIVD
EVLQKSGYRE MLERENTLES RSRLENIDEF MSVPKDYEEN TPLEEQSLIN FLTDLSLVAD
IDEADTENGV TLMTMHSAKG LEFPIVFIMG MEESLFPHIR AIKSEDDHEM QEERRICYVA
ITRAEEVLYI THATSRMLFG RPQSNMPSRF LKEIPESLLE NHSSGKRQTI QPKAKPFAKR
GFSQRSTKKQ VLSSDWNVGD KVMHKAWGEG MVSNVNEKNG SIELDIIFKS QGPKRLLAQF
APIEKKED