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Description:
DNA polymerase I (EC 2.7.7.7) (POL I).
Molecular weight: 10566
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
30-MAY-2000, sequence version 2.
07-MAR-2006, entry version 50.
Phylogenetic order:
Bacteria Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO1_DEIRA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L14581 | AAC36974.1 | - |
| EMBL | AE000513 | AAF11264.1 | - |
| PIR | A40597 | A40597. | |
| HSSP | P19821 | 2KTQ | |
| GenomeReviews | AE000513_GR | DR1707.1 | |
| TIGR | DR1707 | -. | |
| InterPro | IPR002421 | 5_3_exonuclease. | |
| InterPro | IPR001098 | DNA_pol. | |
| InterPro | IPR002298 | DNA_polI. | |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| Pfam | PF01367 | 5_3_exonuc | 1. |
| Pfam | PF02739 | 5_3_exonuc_N | 1. |
| Pfam | PF00476 | DNA_pol_A | 1. |
| PRINTS | PR00868 | DNAPOLI. | |
| SMART | SM00475 | 53EXOc | 1. |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00482 | POLAc | 1. |
| TIGRFAMs | TIGR00593 | pola | 1. |
| PROSITE | PS00447 | DNA_POLYMERASE_A | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 36-956.
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=93273728; PubMed=8501062;
RA Gutman P.D., Fuchs P., Ouyang L., Minton K.W.;
RT "Identification, sequencing, and targeted mutagenesis of a DNA
RT polymerase gene required for the extreme radioresistance of
RT Deinococcus radiodurans.";
RL J. Bacteriol. 175:3581-3590(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=20036896; PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA Fraser C.M.;
RT "Genome sequence of the radioresistant bacterium Deinococcus
RT radiodurans R1.";
RL Science 286:1571-1577(1999).
Feature:
CHAIN 1 956 DNA polymerase I.
/FTId=PRO_0000101238.
CONFLICT 48 48 T -> A (in Ref. 1).
CONFLICT 77 77 H -> D (in Ref. 1).
CONFLICT 108 108 F -> L (in Ref. 1).
CONFLICT 128 128 P -> R (in Ref. 1).
CONFLICT 406 406 P -> R (in Ref. 1).
CONFLICT 540 540 L -> V (in Ref. 1).
Comments:
-!- FUNCTION: In addition to polymerase activity, this DNA polymerase
5' to 3' exonuclease activity (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBUNIT: Single-chain monomer with multiple functions.
-!- SIMILARITY: Belongs to the DNA polymerase type-A family.
-!- SIMILARITY: Contains 1 5'-3' exonuclease domain.
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Sequence length: 956
MVFCGDGGLS CESIDFALCC LRGRSGNYVQ SRILPMADAS PDPSKPDTLV LIDGHALAFR
SYFALPPLNN SKGEMTHAIV GFMKLLLRLA RQKSNQVIVV FDPPVKTFRH EQYEGYKSGR
AQTPEDLPGQ INRIRALVDA LGFPRLEEPG YEADDVIASL TRMAEGKGYE VRIVTSDRDA
YQLLDEHVKV IANDFSLIGP AQVEEKYGVT VRQWVDYRAL TGDASDNIPG AKGIGPKTAA
KLLQEYGTLE KVYEAAHAGT LKPDGTRKKL LDSEENVKFS HDLSCMVTDL PLDIEFGVRR
LPDNPLVTED LLTELELHSL RPMILGLNGP EQDGHAPDDL LEREHAQTPE EDEAAALPAF
SAPELAEWQT PAEGAVWGYV LSREDDLTAA LLAAATFEDG VARPAPVSEP DEWAQAEAPE
NLFGELLPSD KPLTKKEQKA LEKAQKDAEK ARAKLREQFP ATVDEAEFVG QRTVTAAAAK
ALAAHLSVRG TVVEPGDDPL LYAYLLDPAN TNMPVVAKRY LDREWPADAP TRAAITGHLL
RELPPLLDDA RRKMYDEMEK PLSGVLGRME VRGVQVDSDF LQTLSIQAGV RLADLESQIH
EYAGEEFHIR SPKQLETVLY DKLELASSKK TKLTGQRSTA VSALEPLRDA HPIIPLVLEF
RELDKLRGTY LDPIPNLVNP HTGRLHTTFA QTAVATGRLS SLNPNLQNIP IRSELGREIR
KGFIAEDGFT LIAADYSQIE LRLLAHIADD PLMQQAFVEG ADIHRRTAAQ VLGLDEATVD
ANQRRAAKTV NFGVLYGMSA HRLSNDLGIP YAEAATFIEI YFATYPGIRR YINHTLDFGR
THGYVETLYG RRRYVPGLSS RNRVQREAEE RLAYNMPIQG TAADIMKLAM VQLDPQLDAI
GARMLLQVHD ELLIEAPLDK AEQVAALTKK VMENVVQLKV PLAVEVGTGP NWFDTK