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Description:
Holliday junction ATP-dependent DNA helicase ruvB (EC 3.6.1.-).
Molecular weight: 36325
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
30-MAY-2000, sequence version 2.
07-MAR-2006, entry version 41.
Phylogenetic order:
Bacteria Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVB_DEIRA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D84298 | BAA76366.1 | - |
| EMBL | AE000513 | AAF10176.1 | - |
| PIR | D75498 | D75498. | |
| HSSP | Q56214 | 1IXS | |
| SMR | Q9X719 | 10-321.1 | |
| GenomeReviews | AE000513_GR | DR0596.1 | |
| TIGR | DR0596 | -. | |
| HAMAP | MF_00016 | - | 1. |
| InterPro | IPR003593 | AAA_ATPase. | |
| InterPro | IPR003959 | AAA_ATPase_centr. | |
| InterPro | IPR004605 | RuvB. | |
| InterPro | IPR008823 | RuvB_C. | |
| InterPro | IPR008824 | RuvB_N. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF00004 | AAA | 1. |
| Pfam | PF05491 | RuvB_C | 1. |
| Pfam | PF05496 | RuvB_N | 1. |
| SMART | SM00382 | AAA | 1. |
| TIGRFAMs | TIGR00635 | ruvB | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; Helicase; Hydrolase; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=98108493; PubMed=9447236; DOI=10.1016/S0921-8777(97)00048-7;
RA Kitayama S., Kohoroku M., Takagi A., Itoh H.;
RT "Mutation of D. radiodurans in a gene homologous to ruvB of E. coli.";
RL Mutat. Res. 385:151-157(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=20036896; PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA Fraser C.M.;
RT "Genome sequence of the radioresistant bacterium Deinococcus
RT radiodurans R1.";
RL Science 286:1571-1577(1999).
Feature:
CHAIN 1 333 Holliday junction ATP-dependent DNA
helicase ruvB.
/FTId=PRO_0000165526.
NP_BIND 50 57 ATP (Potential).
CONFLICT 87 87 Missing (in Ref. 1).
CONFLICT 162 163 SR -> RA (in Ref. 1).
CONFLICT 214 235 RLLRRVRDYADVAGETTIGLER -> ACCGACATTPTWRAK
PPSAWN (in Ref. 1).
CONFLICT 251 266 DDRDKKYLETLIHRFA -> GTTRQEVPRNPDSPLC (in
Ref. 1).
CONFLICT 303 333 TPRGRVATAHAYDHLGLPPGGIDDGNGIFLN -> STWPRG
DGTRL (in Ref. 1).
Comments:
-!- FUNCTION: The ruvA-ruvB complex in the presence of ATP renatures
cruciform structure in supercoiled DNA with palindromic sequence,
indicating that it may promote strand exchange reactions in
homologous recombination. RuvAB is an helicase that mediates the
Holliday junction migration by localized denaturation and
reannealing (By similarity).
-!- SUBUNIT: Forms a complex with ruvA (By similarity).
-!- SIMILARITY: Belongs to the ruvB family.
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Sequence length: 333
MTAPENLDAA LRPKTLTEYV GQEKLKDKLG VYLQAARGRR EALDHTLLFG PPGLGKTTLA
HIIAYELGVN IRVTSGPAIE KPGDLAAILT NSLEEGDVLF IDEIHRLGRV AEEHLYPAME
DFKLDIVLGQ GPAARTIELP LPRFTLVGAT TRPGLITAPM RSRFGIIEHL EYYTAEEIAT
NLLRDARLLG FGLDEEAGLE IGARSRGTMR IAKRLLRRVR DYADVAGETT IGLERAQSAL
DKLGLDSAGL DDRDKKYLET LIHRFAGGPV GVDTLATAIS EDALTLEDVY EPYLIQLGFI
KRTPRGRVAT AHAYDHLGLP PGGIDDGNGI FLN