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Description:
Single-stranded DNA-binding protein (SSB) (Helix-destabilizingprotein).
Molecular weight: 32722
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
03-OCT-2003, sequence version 2.
07-MAR-2006, entry version 35.
Phylogenetic order:
Bacteria Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein SSB_DEIRA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AJ564860 | CAD92322.1 | - |
| EMBL | AY293617 | AAQ18705.1 | - |
| EMBL | AE000513 | AAF09692.1 | ALT_FRAME |
| PIR | A75559 | A75559. | |
| PDB | 1SE8 | X-ray | A=1-301. |
| GenomeReviews | AE000513_GR | DR0099.1 | |
| TIGR | DR0099 | -. | |
| InterPro | IPR012340 | OB_NA_bd_sub. | |
| InterPro | IPR011344 | Single_strand_bd. | |
| InterPro | IPR000424 | SSB_primosomal_n. | |
| InterPro | IPR010913 | ssDNA_bd. | |
| Pfam | PF00436 | SSB | 2. |
| TIGRFAMs | TIGR00621 | ssb | 2. |
| PROSITE | PS50935 | SSB | 2. |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Repeat.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RA Witte G., Urbanke C., Curth U.;
RT "Single-stranded DNA binding protein from Deinococcus radiodurans -
RT Cloning, expression and biophysical characterization.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RA Eggington J.M., Wood E.A., Cox M.M.;
RT "The Deinococcus radiodurans ssb gene encodes a contiguous ORF for
RT single-stranded DNA-binding protein.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=20036896; PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA Fraser C.M.;
RT "Genome sequence of the radioresistant bacterium Deinococcus
RT radiodurans R1.";
RL Science 286:1571-1577(1999).
Feature:
CHAIN 1 301 Single-stranded DNA-binding protein.
/FTId=PRO_0000096147.
DOMAIN 5 110 SSB 1.
DOMAIN 129 232 SSB 2.
STRAND 5 16
STRAND 19 22
TURN 24 25
STRAND 26 26
STRAND 28 39
STRAND 49 60
HELIX 61 68
TURN 69 69
TURN 73 74
STRAND 76 88
STRAND 96 111
STRAND 115 117
TURN 119 120
STRAND 121 121
STRAND 123 126
TURN 127 127
STRAND 129 138
STRAND 140 140
STRAND 143 146
TURN 148 149
STRAND 152 165
TURN 167 168
STRAND 169 169
STRAND 171 183
TURN 184 184
HELIX 185 190
TURN 191 192
TURN 195 196
STRAND 198 209
STRAND 219 231
Comments:
-!- FUNCTION: This protein is essential for replication of the
chromosome. It is also involved in DNA recombination and repair
(By similarity).
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Contains 2 SSB domains.
-!- CAUTION: Ref.3 sequence differs from that shown due to frameshifts
in positions 93 and 169.
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Sequence length: 301
MARGMNHVYL IGALARDPEL RYTGNGMAVF EATVAGEDRV IGNDGRERNL PWYHRVSILG
KPAEWQAERN LKGGDAVVVE GTLEYRQWEA PEGGKRSAVN VKALRMEQLG TQPELIQDAG
GGVRMSGAMN EVLVLGNVTR DPEIRYTPAG DAVLSLSIAV NENYQDRQGQ RQEKVHYIDA
TLWRDLAENM KELRKGDPVM IMGRLVNEGW TDKDGNKRNS TRVEATRVEA LARGAGNANS
GYAAATPAAP RTQTASSAAR PTSGGYQSQP SRAANTGSRS GGLDIDQGLD DFPPEEDDLP
F