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Description:
UV DNA damage endonuclease (EC 3.-.-.-) (UV-endonuclease) (UVED)(Endonuclease beta).
Molecular weight: 33592
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 ) pyrimidine dimer repair( GO:0006290 )
Important dates:
06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
06-DEC-2002, sequence version 2.
07-MAR-2006, entry version 29.
Phylogenetic order:
Bacteria Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVSE_DEIRA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AB033747 | BAA85759.1 | - |
| EMBL | AE000513 | AAF11370.1 | ALT_INIT |
| GenomeReviews | AE000513_GR | DR1819.1 | |
| TIGR | DR1819 | -. | |
| HAMAP | MF_00606 | - | 1. |
| InterPro | IPR004601 | UvdE. | |
| Pfam | PF03851 | UvdE | 1. |
| TIGRFAMs | TIGR00629 | uvde | 1. |
Keywords:
Complete proteome; DNA damage; DNA excision; DNA repair; Endonuclease; Hydrolase; Manganese; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KR1;
RA Kitayama S., Kikuchi M., Funayama T., Narumi I., Watanabe H.;
RT "Cloning of structural gene of an alternative incision enzyme for DNA
RT damage in Deinococcus radiodurans.";
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=20036896; PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA Fraser C.M.;
RT "Genome sequence of the radioresistant bacterium Deinococcus
RT radiodurans R1.";
RL Science 286:1571-1577(1999).
RN [3]
RP FUNCTION.
RC STRAIN=R1 / ATCC 13939 / DSM 20539 / NCIB 9279;
RX MEDLINE=21666133; PubMed=11807060;
RX DOI=10.1128/jb.184.4.1003-1009.2002;
RA Earl A.M., Rankin S.K., Kim K.-P., Lamendola O.N., Battista J.R.;
RT "Genetic evidence that the uvsE gene product of Deinococcus
RT radiodurans R1 is a UV damage endonuclease.";
RL J. Bacteriol. 184:1003-1009(2002).
RN [4]
RP CHARACTERIZATION.
RX MEDLINE=85163555; PubMed=3838572; DOI=10.1016/0167-8817(85)90018-5;
RA Evans D.M., Moseley B.E.;
RT "Identification and initial characterisation of a pyrimidine dimer UV
RT endonuclease (UV endonuclease beta) from Deinococcus radiodurans; a
RT DNA-repair enzyme that requires manganese ions.";
RL Mutat. Res. 145:119-128(1985).
Feature:
CHAIN 1 305 UV DNA damage endonuclease.
/FTId=PRO_0000215036.
Comments:
-!- FUNCTION: Component in a DNA repair pathway. Removal of UV-light
damaged nucleotides. Recognizes pyrimidine dimers and cleave a
phosphodiester bond immediately 5' to the lesion.
-!- COFACTOR: Manganese.
-!- SIMILARITY: Belongs to the uve1/uvsE family.
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Sequence length: 305
MTSACEAVPQ LGLVCLTVGP EVRFRTVTLS RYRALSPAER EAKLLDLYSS NIKTLRGAAD
YCAAHDIRLY RLSSSLFPML DLAGDDTGAA VLTHLAPQLL EAGHAFTDAG VRLLMHPEQF
IVLNSDRPEV RESSVRAMSA HARVMDGLGL ARTPWNLLLL HGGKGGRGAE LAALIPDLPD
PVRLRLGLEN DERAYSPAEL LPICEATGTP LVFDAHHHVV HDKLPDQEDP SVREWVLRAR
ATWQPPEWQV VHLSNGIEGP QDRRHSHLIA DFPSAYADVP WIEVEAKGKE EAIAALRLMA
PFKKD