Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 34704
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Bacteria Firmicutes Mollicutes Mycoplasmataceae Ureaplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_UREPA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE002128 | AAF30715.1 | - |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | AF222894_GR | UU306.1 | |
| BioCyc | UURE2130:UU306-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700970 / Serovar 3;
RX MEDLINE=20500219; PubMed=11048724; DOI=10.1038/35037619;
RA Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y.,
RA Cassell G.H.;
RT "The complete sequence of the mucosal pathogen Ureaplasma
RT urealyticum.";
RL Nature 407:757-762(2000).
Feature:
CHAIN 1 303 Probable endonuclease IV.
/FTId=PRO_0000190883.
METAL 75 75 Zinc 1 (By similarity).
METAL 115 115 Zinc 1 (By similarity).
METAL 151 151 Zinc 1 (By similarity).
METAL 151 151 Zinc 2 (By similarity).
METAL 185 185 Zinc 2 (By similarity).
METAL 188 188 Zinc 3 (By similarity).
METAL 221 221 Zinc 2 (By similarity).
METAL 234 234 Zinc 3 (By similarity).
METAL 236 236 Zinc 3 (By similarity).
METAL 266 266 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 303
MDKYNLIIGS HVSLKAKDFF YGSVKEALSY GSNTFMVYTG APQNTKRQPI KSFKIEEAHN
LLKKHNINLD DLIVHAPYII NPCSSKKNVR ELAKEFLIQE IQRTESMGIT KLVLHPGSRL
EQNEDIALEQ VYTMLNDIFS TINTNVIVCL ETMAGKGSEI GVNIKQLKTI IDNVHSKKNI
GVCLDTCHMN DSGLILDYYN FNQYLKEFDA QIGINYIKVL HINDSKNPCG ANKDRHENLG
YGTIGFANLI NIIYHPLLNN IPKILETPWF NVNDELIPLY KHEIKMIRDC KWYDIKNKLL
SKK