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Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 47450
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 27.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus Bacillus cereus group.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_BACAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE016879 | AAP28082.1 | - |
| EMBL | AE017334 | AAT33486.1 | - |
| EMBL | AE017225 | AAT56352.1 | - |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | AE016879_GR | BA4366.1 | |
| GenomeReviews | AE017225_GR | BAS4051.1 | |
| GenomeReviews | AE017334_GR | GBAA4366.1 | |
| TIGR | BA4366 | -. | |
| TIGR | GBAA4366 | -. | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX MEDLINE=22608414; PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H.,
RA Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D.,
RA Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F.,
RA Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C.,
RA Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O.,
RA Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to
RT closely related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RA Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B.,
RA Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L.,
RA Fraser C.M.;
RT "Bacillus anthracis comparative genomics.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 412 DNA polymerase IV.
/FTId=PRO_0000173899.
DOMAIN 12 193 UmuC.
ACT_SITE 113 113 By similarity.
METAL 16 16 Magnesium (By similarity).
METAL 112 112 Magnesium (By similarity).
SITE 21 21 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 412
MREMYPKKGR VILHVDMNCF FASVEIAHDS SLQGKPLAVA GNEKERKGII ITCSYEAREY
GIRTTMPLWE AKRLCPQLIV RRPNFTLYRE ASFQMFQILS RFTEKIQPVS IDEGYLDITD
CYALGSPLEI AKMIQQALLT ELQLPCSIGI APNLFLAKTA SDMKKPLGIT VLRKRDIPEM
IWPLPVGAMH GIGEKTAEKL NDIHIQTIEQ LAKGNEHIIR AKIGKHGVDL QRRAKGMDDR
EVDPSQMGQH KSVGNSMTFS KDMDEEKELL DMLQRLSKSV SKRLQKRTLV SYNIQIMIKY
HDRRTVTRSK QLKNAIWEER DIFQAASRLW KQHWDGDSVR LLGVTATEIE WKTESVKQLD
LFSFEEDAKE EPLLAVIDQI NDKYGMPLLQ RGSQLLRKQE KSFQQKLESK FM