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Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 32909
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 25.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus Bacillus cereus group.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_BACAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE016879 | AAP28219.1 | - |
| EMBL | AE017334 | AAT33627.1 | - |
| EMBL | AE017225 | AAT56486.1 | - |
| PDB | 1XP3 | X-ray | A=1-298. |
| GenomeReviews | AE016879_GR | BA4508.1 | |
| GenomeReviews | AE017225_GR | BAS4186.1 | |
| GenomeReviews | AE017334_GR | GBAA4508.1 | |
| TIGR | BA4508 | -. | |
| TIGR | GBAA4508 | -. | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX MEDLINE=22608414; PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H.,
RA Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D.,
RA Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F.,
RA Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C.,
RA Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O.,
RA Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to
RT closely related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RA Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B.,
RA Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L.,
RA Fraser C.M.;
RT "Bacillus anthracis comparative genomics.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 298 Probable endonuclease IV.
/FTId=PRO_0000190819.
METAL 69 69 Zinc 1 (By similarity).
METAL 111 111 Zinc 1 (By similarity).
METAL 146 146 Zinc 1 (By similarity).
METAL 146 146 Zinc 2 (By similarity).
METAL 180 180 Zinc 2 (By similarity).
METAL 183 183 Zinc 3 (By similarity).
METAL 215 215 Zinc 2 (By similarity).
METAL 228 228 Zinc 3 (By similarity).
METAL 230 230 Zinc 3 (By similarity).
METAL 260 260 Zinc 2 (By similarity).
STRAND 4 7
STRAND 12 14
TURN 15 15
HELIX 16 24
TURN 25 27
STRAND 29 33
STRAND 35 36
TURN 38 39
HELIX 46 49
HELIX 51 60
TURN 61 62
STRAND 66 69
TURN 72 73
TURN 76 77
STRAND 80 80
HELIX 82 101
STRAND 102 104
STRAND 107 110
STRAND 113 114
TURN 116 117
STRAND 118 118
HELIX 120 134
TURN 137 138
STRAND 140 146
TURN 152 153
STRAND 154 155
STRAND 158 158
HELIX 159 168
TURN 170 171
HELIX 172 174
STRAND 175 180
HELIX 181 186
TURN 187 188
HELIX 191 205
TURN 206 206
HELIX 208 210
STRAND 211 216
STRAND 218 221
TURN 223 224
STRAND 230 231
TURN 233 234
STRAND 235 238
HELIX 240 247
TURN 248 248
HELIX 250 252
TURN 253 254
STRAND 255 255
STRAND 257 259
STRAND 264 264
STRAND 267 269
STRAND 272 272
HELIX 275 284
TURN 285 285
STRAND 286 286
TURN 289 290
HELIX 291 296
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 298
MLKIGSHVSM SGKKMLLAAS EEAVSYGATT FMIYTGAPQN TRRKPIEELN IEAGRKHMEQ
NGIEEIIVHA PYIINVGNTT KPETFQLGVD FLRMEIERTS ALGVAKQIVL HPGAHVGAGA
DAGIQQIIKG LNEVLTPDQT VNIALETMAG KGTECGRSFE EIAKIIDGVK YNEKLSVCFD
TCHTHDAGYD IVNNFDGVLN EFDKIVGIDR LQVLHINDSK NVRGAGKDRH ENIGFGHIGY
KALHHIVHHP QLTHVPKILE TPYVGEDKKD KKPPYKLEIE MLKNGTFDEG LLEKIKAQ