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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 22890
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 22.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus Bacillus cereus group.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_BACAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE016879 | AAP27500.1 | - |
| EMBL | AE017334 | AAT32868.1 | - |
| EMBL | AE017225 | AAT55785.1 | ALT_INIT |
| HSSP | P03033 | 1LEA | |
| GenomeReviews | AE016879_GR | BA3754.1 | |
| GenomeReviews | AE017225_GR | BAS3479.1 | |
| GenomeReviews | AE017334_GR | GBAA3754.1 | |
| TIGR | BA3754 | -. | |
| TIGR | GBAA3754 | -. | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX MEDLINE=22608414; PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H.,
RA Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D.,
RA Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F.,
RA Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C.,
RA Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O.,
RA Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to
RT closely related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RA Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B.,
RA Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L.,
RA Fraser C.M.;
RT "Bacillus anthracis comparative genomics.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 206 LexA repressor.
/FTId=PRO_0000170002.
DNA_BIND 28 48 H-T-H motif (By similarity).
ACT_SITE 128 128 Involved in auto-cleavage (By
similarity).
ACT_SITE 166 166 Involved in auto-cleavage (By
similarity).
SITE 92 93 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 206
MEKLTKRQQD ILDFIKLKVQ EKGYPPSVRE IGQAVGLASS STVHGHLSRL EEKGYIRRDP
TKPRAIEILG EDRMDTETQS VIQVPIVGKV TAGLPITAVE SVEEHFPLPA SIVAGADQVF
MLRISGDSMI EAGIFDGDLV VVRQQQSAYN GEIVVALTED NEATVKRFYK EKDHFRLQPE
NSSLEPIILK QVSVIGKVIG VYRDLH