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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 75317
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-JUN-1998, sequence version 1.
07-MAR-2006, entry version 41.
Phylogenetic order:
Bacteria Spirochaetes Spirochaetales Spirochaetaceae Borrelia Borrelia burgdorferi group.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_BORBU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE001157 | AAC66923.1 | - |
| PIR | G70168 | G70168. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | AE000783_GR | BB0552.1 | |
| TIGR | BB0552 | -. | |
| BioCyc | BBUR139:BB0552-MONOMER | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00292 | BRCT | 1. |
| SMART | SM00278 | HhH1 | 1. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35210 / B31;
RX MEDLINE=98065943; PubMed=9403685; DOI=10.1038/37551;
RA Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K.,
RA Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D.,
RA Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J.,
RA Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D.,
RA Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A.,
RA Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K.,
RA Roberts K.M., Hatch B., Smith H.O., Venter J.C.;
RT "Genomic sequence of a Lyme disease spirochaete, Borrelia
RT burgdorferi.";
RL Nature 390:580-586(1997).
Feature:
CHAIN 1 660 DNA ligase.
/FTId=PRO_0000161739.
DOMAIN 583 660 BRCT.
ACT_SITE 112 112 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA (By similarity).
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
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Sequence length: 660
MSSKVQQEIA DLKKLIRKWD KEYYVDSLPS VEDFVYDKHI LRLQELESKY PEYKTLDSPT
LKFGSDLLND FKEVEHSAPI LSLDKVYDLD LLKSWIDKID FNNSFNISVE PKIDGCSIVL
YYKDGVLEKA LTRGNGKFGN DVTINVRTIR YIPLFLDEKV DLVLRGEVYI TKENFLKINK
FLEKPYTNSR NLASGILRRV DSREVANFPL NIFIYDFLNA GLEFKTNDLA TARLKKLGFK
VNPLIRFFDL KNSIGEVLNY IADITKKRDS FEYEIDGVVL KVSDFALRER LGYTAHHPKW
AMAYKFEALS GFSRVNSIVV QVGRSGKITP VANIDKVFVS GAFITSATLH NQDYIRSIGL
NVGDVVKVSR RGDVIPAVEM VINKFSTGFF KVPDNCPACK TAVVKEGAHF FCPNNNCPSV
AVERIKYFCS KNCMDIEGFS DKIISFLFEK KFIFSEIDLY TFDFYKLLEF KGFKDRKINN
LINSIEASKK KPFSKLLLSM GIKDLGENTI RLLFLNNLNS FSKLFKLCQD RYFAFSTLLK
IKGIGEKIAL NIIEAFNDSV MLNKFKFFEN LEFKMEEVVA IDGENKLLAG KKFCITGTFN
GYSRSIIIDK LKNKGAIFNT CVTGSLDFLI VGEKAGSKLK KALSLNIKIM SFEDIKSYLD