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Description:
DNA-3-methyladenine glycosylase (EC 3.2.2.21) (3-methyladenine-DNAglycosidase).
Molecular weight: 35634
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 1.
07-MAR-2006, entry version 44.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein 3MGA_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D14465 | BAA03361.1 | - |
| EMBL | AB006424 | BAA33073.1 | - |
| EMBL | Z99104 | CAB11956.1 | - |
| PIR | E69584 | E69584. | |
| GenomeReviews | AL009126_GR | BSU01800.1 | |
| SubtiList | BG10165 | alkA. | |
| BioCyc | BSUB1423:BSU0180-MONOMER | -.1 | |
| InterPro | IPR000035 | AlbDNA_glycsylse. | |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR012904 | OGG_N. | |
| InterPro | IPR012294 | TFIID_C/glycos_N. | |
| Pfam | PF00730 | HhH-GPD | 1. |
| Pfam | PF07934 | OGG_N | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| PROSITE | PS00516 | ALKYLBASE_DNA_GLYCOS | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Hydrolase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=93388534; PubMed=8376346;
RA Morohoshi F., Hayashi K., Munakata N.;
RT "Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA
RT glycosylase is adjacent to the ada operon.";
RL J. Bacteriol. 175:6010-6017(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT Bacillus subtilis chromosome.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
Feature:
CHAIN 1 303 DNA-3-methyladenine glycosylase.
/FTId=PRO_0000194880.
Comments:
-!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to
excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-
methylthymine, and O2-methylcytosine from the damaged DNA polymer
formed by alkylation lesions.
-!- CATALYTIC ACTIVITY: Hydrolysis of alkylated DNA, releasing 3-
methyladenine, 3-methylguanine, 7-methylguanine and 7-
methyladenine.
-!- SIMILARITY: Belongs to the alkylbase DNA glycosidase alkA family.
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Sequence length: 303
MTWHEVNDVI VITLPEIFDM NANLGYLTRE KNECMYEIEN NIITKVIAIG EIRSLVQVSV
INNKQMIVQF LNDSRPVEQW KREEIVKYIH EWFDLDNDLT PFYEMAKADP LLKMPARKFY
GLRVIGIPDL FEALCWGVLG QQINLAFAYS LKKQFVEAFG DSIEWNGKKY WVFPPYERIA
RLTPTDLADI KMTVKKSEYI IGIARLMASG ELSREKLMKM NFKDAEKNLI KIRGIGPWTA
NYVLMRCLRF PTAFPIDDVG LIHSIKILRN MNRKPTKDEI LEISVPWKEW QSYATFYLWR
VLY