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Description:
Methylphosphotriester-DNA alkyltransferase.
Molecular weight: 24299
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
01-NOV-1990, sequence version 1.
07-MAR-2006, entry version 52.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ADAA_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X53399 | CAA37475.1 | - |
| EMBL | AB006424 | BAA33074.1 | - |
| EMBL | Z99104 | CAB11957.1 | - |
| PIR | S11483 | XUBSMM. | |
| HSSP | P06134 | 1ADN | |
| GenomeReviews | AL009126_GR | BSU01810.1 | |
| SubtiList | BG10166 | adaA. | |
| BioCyc | BSUB1423:BSU0181-MONOMER | -.1 | |
| LinkHub | P19219 | -.1 | |
| InterPro | IPR004026 | Ada_Zn_bd. | |
| InterPro | IPR012287 | Homeodomain-rel. | |
| InterPro | IPR000005 | HTHAraC. | |
| Pfam | PF02805 | Ada_Zn_binding | 1. |
| Pfam | PF00165 | HTH_AraC | 2. |
| PRINTS | PR00032 | HTHARAC. | |
| SMART | SM00342 | HTH_ARAC | 1. |
| PROSITE | PS00041 | HTH_ARAC_FAMILY_1 | 1. |
| PROSITE | PS01124 | HTH_ARAC_FAMILY_2 | 1. |
Keywords:
Activator; Complete proteome; DNA damage; DNA repair; DNA-binding; Metal-binding; Methyltransferase; Transcription; Transcription regulation; Transferase; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=91016831; PubMed=2120677;
RA Morohoshi F., Hayashi K., Munakata N.;
RT "Bacillus subtilis ada operon encodes two DNA alkyltransferases.";
RL Nucleic Acids Res. 18:5473-5480(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT Bacillus subtilis chromosome.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP MUTAGENESIS.
RX MEDLINE=92078089; PubMed=1744039;
RA Morohoshi F., Hayashi K., Munakata N.;
RT "Molecular analysis of Bacillus subtilis ada mutants deficient in the
RT adaptive response to simple alkylating agents.";
RL J. Bacteriol. 173:7834-7840(1991).
Feature:
CHAIN 1 211 Methylphosphotriester-DNA
alkyltransferase.
/FTId=PRO_0000194494.
DNA_BIND 118 137 H-T-H motif (By similarity).
ACT_SITE 85 85 Acceptor for methyl from phosphotriester.
METAL 54 54 Zinc (By similarity).
METAL 58 58 Zinc (By similarity).
METAL 85 85 Zinc (By similarity).
METAL 88 88 Zinc (By similarity).
MUTAGEN 86 87 KR->NC: Loss of activity.
Comments:
-!- FUNCTION: Methylphosphotriester-DNA alkyltransferase is one of two
genes required for the adaptive response of bacteria to alkylating
agents. It accepts a methyl group from methylphospho-triesters and
then acts as a transcriptional activator of the ada operon.
-!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
-!- SIMILARITY: Contains 1 HTH araC/xylS-type DNA-binding domain.
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Sequence length: 211
MPDSINNGHK ESHDHRISND AEMITDEKWQ AIINNDAAYN NQFFYAVKST GIFCKPSCKS
RVPKKENVCI FPNTEQALRA NFRPCKRCKP TNEKMPDSEW VDLITEYIDK NFTEKLTLES
LADICHGSPY HMHRTFKKIK GITLVEYIQQ VRVHAAKKYL IQTNKAIGDI AICVGIANAP
YFITLFKKKT GQTPARFRQM SKMEETYNGN K