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Description:
ATP-dependent nuclease subunit A (EC 3.6.1.-) (ATP-dependent helicaseaddA).
Molecular weight: 1410
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
01-NOV-1991, sequence version 1.
07-MAR-2006, entry version 45.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ADDA_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M63489 | AAA22201.1 | - |
| EMBL | Y14081 | CAA74482.1 | - |
| EMBL | Z99109 | CAB12903.1 | - |
| EMBL | Y09476 | CAA70668.1 | - |
| PIR | B39432 | B39432. | |
| HSSP | P56255 | 1PJR | |
| GenomeReviews | AL009126_GR | BSU10630.1 | |
| SubtiList | BG10466 | addA. | |
| BioCyc | BSUB1423:BSU1063-MONOMER | -.1 | |
| InterPro | IPR000212 | UvrD-helicase. | |
| PANTHER | PTHR11070 | UvrD-helicase.1 | 2. |
| Pfam | PF00580 | UvrD-helicase | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA repair; Endonuclease; Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=OG1;
RX MEDLINE=91267926; PubMed=1646786;
RA Kooistra J., Venema G.;
RT "Cloning, sequencing, and expression of Bacillus subtilis genes
RT involved in ATP-dependent nuclease synthesis.";
RL J. Bacteriol. 173:3644-3655(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98240224; PubMed=9579061;
RA Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R.,
RA Wedler H., Venema G., Bron S.;
RT "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the
RT Bacillus subtilis chromosome contains several dysfunctional genes, the
RT glyB marker, many genes encoding transporter proteins, and the
RT ubiquitous hit gene.";
RL Microbiology 144:859-875(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1000-1232.
RC STRAIN=168;
RX MEDLINE=98015415; PubMed=9353931;
RA Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J.;
RT "Sequencing of regions downstream of addA (98 degrees) and citG (289
RT degrees) in Bacillus subtilis.";
RL Microbiology 143:3305-3308(1997).
Feature:
CHAIN 1 1232 ATP-dependent nuclease subunit A.
/FTId=PRO_0000064449.
NP_BIND 30 37 ATP (Potential).
Comments:
-!- FUNCTION: The enzyme complex may have a wide variety of catalytic
activities including ATP-dependent exonuclease, ATP-stimulated
endonuclease, ATP-dependent helicase and DNA-dependent ATPase
activities. The subunit A may have a role in DNA unwinding
(helicase), and in the nuclease activity (By similarity).
-!- SUBUNIT: The B.subtilis ATP-dependent nuclease complex is formed
by one B subunit in conjunction with one A subunit.
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Sequence length: 1232
MNIPKPADST WTDDQWNAIV STGQDILVAA AAGSGKTAVL VERMIRKITA EENPIDVDRL
LVVTFTNASA AEMKHRIAEA LEKELVQRPG SLHIRRQLSL LNRASISTLH SFCLQVLKKY
YYLIDLDPGF RIADQTEGEL IGDEVLDELF EDEYAKGEKA FFELVDRYTT DRHDLDLQFL
VKQVYEYSRS HPNPEAWLES FVHLYDVSEK SAIEELPFYQ YVKEDIAMVL NGAKEKLLRA
LELTKAPGGP APRADNFLDD LAQIDELIQH QDDFSELYKR VPAVSFKRAK AVKGDEFDPA
LLDEATDLRN GAKKLLEKLK TDYFTRSPEQ HLKSLAEMKP VIETLVQLVI SYGKRFEAAK
QEKSIIDFSD LEHYCLAILT AENDKGEREP SEAARFYQEQ FHEVLVDEYQ DTNLVQESIL
QLVTSGPEET GNLFMVGDVK QSIYRFRLAE PLLFLSKYKR FTESGEGTGR KIDLNKNFRS
RADILDSTNF LFKQLMGGKI GEVDYDEQAE LKLGAAYPDN DETETELLLI DNAEDTDASE
EAEELETVQF EAKAIAKEIR KLISSPFKVY DGKKKTHRNI QYRDIVILLR SMPWAPQIME
ELRAQGIPVY ANLTSGYFEA VEVAVALSVL KVIDNPYQDI PLASVLRSPI VGADENELSL
IRLENKKAPY YEAMKDYLAA GDRSDELYQK LNTFYGHLQK WRAFSKNHSV SELIWEVYRD
TKYMDYVGGM PGGKQRQANL RVLYDRARQY ESTAFRGLFR FLRFIERMQE RGDDLGTARG
LSEQEDVVRL MTIHSSKGLE FPVVFVAGLG RNFNMMDLNK SYLLDKELGF GTKYIHPQLR
ISYPTLPLIA MKKKMRRELL SEELRVLYVA LTRAKEKLFL IGSCKDHQKQ LAKWQASASQ
TDWLLPEFDR YQARTYLDFI GPALARHRDL GDLAGVPAHA DISGHPARFA VQMIHSYDLL
DDDLEERMEE KSERLEAIRR GEPVPGSFAF DEKAREQLSW TYPHQEVTQI RTKQSVSEIK
RKREYEDEYS GRAPVKPADG SILYRRPAFM MKKGLTAAEK GTAMHTVMQH IPLSHVPSIE
EAEQTVHRLY EKELLTEEQK DAIDIEEIVQ FFHTEIGGQL IGAKWKDREI PFSLALPAKE
IYPDAHEADE PLLVQGIIDC LYETEDGLYL LDYKSDRIEG KFQHGFEGAA PILKKRYETQ
IQLYTKAVEQ IAKTKVKGCA LYFFDGGHIL TL