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Description:
Negative regulator of genetic competence clpC/mecB.
Molecular weight: 90119
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 1.
07-MAR-2006, entry version 49.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein CLPC_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D26185 | BAA05320.1 | - |
| EMBL | U02604 | AAA19233.1 | - |
| EMBL | Z99104 | CAB11862.1 | - |
| EMBL | X75930 | CAA53534.1 | - |
| PIR | I40508 | I40508. | |
| HSSP | P03815 | 1JBK | |
| GenomeReviews | AL009126_GR | BSU00860.1 | |
| SubtiList | BG10148 | clpC. | |
| BioCyc | BSUB1423:BSU0086-MONOMER | -.1 | |
| InterPro | IPR013093 | AAA_2. | |
| InterPro | IPR003593 | AAA_ATPase. | |
| InterPro | IPR003959 | AAA_ATPase_centr. | |
| InterPro | IPR001270 | Chaprnin_clpA/B. | |
| InterPro | IPR004176 | Clp_N. | |
| InterPro | IPR001943 | UvrB/C. | |
| Pfam | PF00004 | AAA | 1. |
| Pfam | PF07724 | AAA_2 | 1. |
| Pfam | PF02861 | Clp_N | 2. |
| Pfam | PF02151 | UVR | 1. |
| PRINTS | PR00300 | CLPPROTEASEA. | |
| SMART | SM00382 | AAA | 2. |
| PROSITE | PS00870 | CLPAB_1 | 1. |
| PROSITE | PS00871 | CLPAB_2 | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Chaperone; Competence; Complete proteome; Nucleotide-binding; Repeat; Repressor; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg;
RX MEDLINE=94286523; PubMed=8016066;
RA Msadek T., Kunst F., Rapoport G.;
RT "MecB of Bacillus subtilis, a member of the ClpC ATPase family, is a
RT pleiotropic regulator controlling competence gene expression and
RT growth at high temperature.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:5788-5792(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=96051385; PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus
RT subtilis chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-387.
RX MEDLINE=94253006; PubMed=8195092;
RA Krueger E., Voelker U., Hecker M.;
RT "Stress induction of clpC in Bacillus subtilis and its involvement in
RT stress tolerance.";
RL J. Bacteriol. 176:3360-3367(1994).
Feature:
CHAIN 1 810 Negative regulator of genetic competence
clpC/mecB.
/FTId=PRO_0000191228.
REPEAT 19 50 1.
REPEAT 93 124 2.
DOMAIN 417 452 UVR.
NP_BIND 208 215 ATP (Potential).
NP_BIND 545 552 ATP (Potential).
REGION 19 124 2 X 32 AA approximate repeats.
REGION 163 410 I.
REGION 471 662 II.
Comments:
-!- FUNCTION: Competence gene repressor; required for cell growth at
high temperature. Negative regulator of comK expression. May
interact with mecA to negatively regulate comK.
-!- SIMILARITY: Belongs to the clpA/clpB family. ClpC subfamily.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 810
MMFGRFTERA QKVLALAQEE ALRLGHNNIG TEHILLGLVR EGEGIAAKAL QALGLGSEKI
QKEVESLIGR GQEMSQTIHY TPRAKKVIEL SMDEARKLGH SYVGTEHILL GLIREGEGVA
ARVLNNLGVS LNKARQQVLQ LLGSNETGSS AAGTNSNANT PTLDSLARDL TAIAKEDSLD
PVIGRSKEIQ RVIEVLSRRT KNNPVLIGEP GVGKTAIAEG LAQQIINNEV PEILRDKRVM
TLDMGTVVAG TKYRGEFEDR LKKVMDEIRQ AGNIILFIDE LHTLIGAGGA EGAIDASNIL
KPSLARGELQ CIGATTLDEY RKYIEKDAAL ERRFQPIQVD QPSVDESIQI LQGLRDRYEA
HHRVSITDDA IEAAVKLSDR YISDRFLPDK AIDLIDEAGS KVRLRSFTTP PNLKELEQKL
DEVRKEKDAA VQSQEFEKAA SLRDTEQRLR EQVEDTKKSW KEKQGQENSE VTVDDIAMVV
SSWTGVPVSK IAQTETDKLL NMENILHSRV IGQDEAVVAV AKAVRRARAG LKDPKRPIGS
FIFLGPTGVG KTELARALAE SIFGDEESMI RIDMSEYMEK HSTSRLVGSP PGYVGYDEGG
QLTEKVRRKP YSVVLLDEIE KAHPDVFNIL LQVLEDGRLT DSKGRTVDFR NTILIMTSNV
GASELKRNKY VGFNVQDETQ NHKDMKDKVM GELKRAFRPE FINRIDEIIV FHSLEKKHLT
EIVSLMSDQL TKRLKEQDLS IELTDAAKAK VAEEGVDLEY GARPLRRAIQ KHVEDRLSEE
LLRGNIHKGQ HIVLDVEDGE FVVKTTAKTN