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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 74876
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
07-MAR-2006, entry version 49.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | Y15254 | NOT_ANNOTATED_CDS | |
| EMBL | Z99107 | CAB12482.1 | - |
| PIR | F69794 | F69794. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | AL009126_GR | BSU06620.1 | |
| SubtiList | BG12835 | ligA. | |
| BioCyc | BSUB1423:BSU0663-MONOMER | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| Pfam | PF00633 | HHH | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00292 | BRCT | 1. |
| SMART | SM00278 | HhH1 | 1. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | 1. |
| PROSITE | PS01055 | DNA_LIGASE_N1 | 1. |
| PROSITE | PS01056 | DNA_LIGASE_N2 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98367141; PubMed=9701819;
RA Petit M.-A., Dervyn E., Rose M., Entian K.-D., McGovern S.,
RA Ehrlich S.D., Bruand C.;
RT "PcrA is an essential DNA helicase of Bacillus subtilis fulfilling
RT functions both in repair and rolling-circle replication.";
RL Mol. Microbiol. 29:261-273(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
Feature:
CHAIN 1 668 DNA ligase.
/FTId=PRO_0000161738.
DOMAIN 590 668 BRCT.
ACT_SITE 115 115 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA (By similarity).
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
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Sequence length: 668
MDKETAKQRA EELRRTINKY SYEYYTLDEP SVPDAEYDRL MQELIAIEEE HPDLRTPDSP
TQRVGGAVLE AFQKVTHGTP MLSLGNAFNA DDLRDFDRRV RQSVGDDVAY NVELKIDGLA
VSLRYEDGYF VRGATRGDGT TGEDITENLK TIRNIPLKMN RELSIEVRGE AYMPKRSFEA
LNEERIKNEE EPFANPRNAA AGSLRQLDPK IAAKRNLDIF VYSIAELDEM GVETQSQGLD
FLDELGFKTN QERKKCGSIE EVITLIDELQ AKRADLPYEI DGIVIKVDSL DQQEELGFTA
KSPRWAIAYK FPAEEVVTKL LDIELNVGRT GVITPTAILE PVKVAGTTVS RASLHNEDLI
KEKDIRILDK VVVKKAGDII PEVVNVLVDQ RTGEEKEFSM PTECPECGSE LVRIEGEVAL
RCINPECPAQ IREGLIHFVS RNAMNIDGLG ERVITQLFEE NLVRNVADLY KLTKERVIQL
ERMGEKSTEN LISSIQKSKE NSLERLLFGL GIRFIGSKAA KTLAMHFESL ENLKKASKEE
LLAVDEIGEK MADAVITYFH KEEMLELLNE LQELGVNTLY KGPKKVKAED SDSYFAGKTI
VLTGKLEELS RNEAKAQIEA LGGKLTGSVS KNTDLVIAGE AAGSKLTKAQ ELNIEVWNEE
QLMGELKK