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Description:
DNA polymerase I (EC 2.7.7.7) (POL I).
Molecular weight: 99092
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
07-MAR-2006, entry version 52.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO1_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF008220 | AAC00350.1 | - |
| EMBL | Z99118 | CAB14869.1 | - |
| PIR | E69680 | E69680. | |
| HSSP | P52026 | 1L3T | |
| GenomeReviews | AL009126_GR | BSU29090.1 | |
| SubtiList | BG12656 | polA. | |
| BioCyc | BSUB1423:BSU2905-MONOMER | -.1 | |
| InterPro | IPR002562 | 3_5_exonuclease. | |
| InterPro | IPR002421 | 5_3_exonuclease. | |
| InterPro | IPR001098 | DNA_pol. | |
| InterPro | IPR002298 | DNA_polI. | |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR012337 | RNaseH_fold. | |
| Pfam | PF01367 | 5_3_exonuc | 1. |
| Pfam | PF02739 | 5_3_exonuc_N | 1. |
| Pfam | PF00476 | DNA_pol_A | 1. |
| PRINTS | PR00868 | DNAPOLI. | |
| SMART | SM00474 | 35EXOc | 1. |
| SMART | SM00475 | 53EXOc | 1. |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00482 | POLAc | 1. |
| TIGRFAMs | TIGR00593 | pola | 1. |
| PROSITE | PS00447 | DNA_POLYMERASE_A | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98048467; PubMed=9387221;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.-D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes
RT in the 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
Feature:
CHAIN 1 880 DNA polymerase I.
/FTId=PRO_0000101235.
Comments:
-!- FUNCTION: In addition to polymerase activity, this DNA polymerase
exhibits 3' to 5' and 5' to 3' exonuclease activity (By
similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBUNIT: Single-chain monomer with multiple functions.
-!- SIMILARITY: Belongs to the DNA polymerase type-A family.
-!- SIMILARITY: Contains 1 3'-5' exonuclease domain.
-!- SIMILARITY: Contains 1 5'-3' exonuclease domain.
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Sequence length: 880
MTERKKLVLV DGNSLAYRAF FALPLLSNDK GVHTNAVYGF AMILMKMLED EKPTHMLVAF
DAGKTTFRHG TFKEYKGGRQ KTPPELSEQM PFIRELLDAY QISRYELEQY EADDIIGTLA
KSAEKDGFEV KVFSGDKDLT QLATDKTTVA ITRKGITDVE FYTPEHVKEK YGLTPEQIID
MKGLMGDSSD NIPGVPGVGE KTAIKLLKQF DSVEKLLESI DEVSGKKLKE KLEEFKDQAL
MSKELATIMT DAPIEVSVSG LEYQGFNREQ VIAIFKDLGF NTLLERLGED SAEAEQDQSL
EDINVKTVTD VTSDILVSPS AFVVEQIGDN YHEEPILGFS IVNETGAYFI PKDIAVESEV
FKEWVENDEQ KKWVFDSKRA VVALRWQGIE LKGAEFDTLL AAYIINPGNS YDDVASVAKD
YGLHIVSSDE SVYGKGAKRA VPSEDVLSEH LGRKALAIQS LREKLVQELE NNDQLELFEE
LEMPLALILG EMESTGVKVD VDRLKRMGEE LGAKLKEYEE KIHEIAGEPF NINSPKQLGV
ILFEKIGLPV VKKTKTGYST SADVLEKLAD KHDIVDYILQ YRQIGKLQST YIEGLLKVTR
PDSHKVHTRF NQALTQTGRL SSTDPNLQNI PIRLEEGRKI RQAFVPSEKD WLIFAADYSQ
IELRVLAHIS KDENLIEAFT NDMDIHTKTA MDVFHVAKDE VTSAMRRQAK AVNFGIVYGI
SDYGLSQNLG ITRKEAGAFI DRYLESFQGV KAYMEDSVQE AKQKGYVTTL MHRRRYIPEL
TSRNFNIRSF AERTAMNTPI QGSAADIIKK AMIDMAAKLK EKQLKARLLL QVHDELIFEA
PKEEIEILEK LVPEVMEHAL ALDVPLKVDF ASGPSWYDAK