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Description:
Probable endonuclease III (EC 4.2.99.18) (DNA-(apurinic orapyrimidinic site) lyase).
Molecular weight: 25000
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
01-FEB-1995, sequence version 1.
07-MAR-2006, entry version 51.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END3_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U11289 | AAA80005.1 | - |
| EMBL | L47709 | AAB38457.1 | - |
| EMBL | Z99115 | CAB14150.1 | - |
| PIR | I40525 | I40525. | |
| HSSP | P20625 | 2ABK | |
| SMR | P39788 | 1-214.1 | |
| GenomeReviews | AL009126_GR | BSU22340.1 | |
| SubtiList | BG10956 | nth. | |
| BioCyc | BSUB1423:BSU2233-MONOMER | -.1 | |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR004035 | EndoIII_FCL. | |
| InterPro | IPR004036 | EndoIII_HhH. | |
| InterPro | IPR003651 | FeS_bind. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR005759 | Nth. | |
| Pfam | PF00633 | HHH | 1. |
| Pfam | PF00730 | HhH-GPD | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| SMART | SM00525 | FES | 1. |
| TIGRFAMs | TIGR01083 | nth | 1. |
| PROSITE | PS00764 | ENDONUCLEASE_III_1 | 1. |
| PROSITE | PS01155 | ENDONUCLEASE_III_2 | 1. |
Keywords:
4Fe-4S; Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase; Iron; Iron-sulfur; Lyase; Metal-binding; Multifunctional enzyme.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=95219085; PubMed=7704260;
RA Bruand C., Sorokin A., Serror P., Ehrlich S.D.;
RT "Nucleotide sequence of the Bacillus subtilis dnaD gene.";
RL Microbiology 141:321-322(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg;
RX MEDLINE=96349105; PubMed=8760912;
RA Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D.,
RA Serror P.;
RT "Sequence analysis of the Bacillus subtilis chromosome region between
RT the serA and kdg loci cloned in a yeast artificial chromosome.";
RL Microbiology 142:2005-2016(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
Feature:
CHAIN 1 219 Probable endonuclease III.
/FTId=PRO_0000102213.
METAL 189 189 Iron-sulfur (4Fe-4S) (By similarity).
METAL 196 196 Iron-sulfur (4Fe-4S) (By similarity).
METAL 199 199 Iron-sulfur (4Fe-4S) (By similarity).
METAL 205 205 Iron-sulfur (4Fe-4S) (By similarity).
Comments:
-!- CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or
apyrimidinic site in DNA is broken by a beta-elimination reaction,
leaving a 3'-terminal unsaturated sugar and a product with a
terminal 5'-phosphate.
-!- COFACTOR: Binds 1 4Fe-4S cluster which is not important for the
catalytic activity, but which is probably involved in the proper
positioning of the enzyme along the DNA strand (By similarity).
-!- SIMILARITY: Belongs to the nth/mutY family.
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Sequence length: 219
MLNLKQIEFC LDKIGDMFPH AECELVHSNP FELVVAVALS AQCTDALVNR VTKTLFQKYK
RPEDYLAVPL EELQQDIKSI GLYRNKAKNI QKLSKMIIED YGGEVPRDRD ELVKLPGVGR
KTANVVVSVA FGVPAIAVDT HVERVSKRLG ICRWKDSVLE VEKTLMRKVP KEDWSVTHHR
LIFFGRYHCK AQSPRCAECP LLSLCREGQK RDKKGLVKR