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Description:
Transcription-repair coupling factor (EC 3.6.1.-) (TRCF) (ATP-dependent helicase mfd).
Molecular weight: 1338
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 1.
07-MAR-2006, entry version 49.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MFD_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D26185 | BAA05290.1 | - |
| EMBL | Z99104 | CAB11831.1 | - |
| PIR | S66085 | S66085. | |
| HSSP | Q9WY48 | 1GM5 | |
| GenomeReviews | AL009126_GR | BSU00550.1 | |
| SubtiList | BG10118 | mfd. | |
| BioCyc | BSUB1423:BSU0055-MONOMER | -.1 | |
| InterPro | IPR003711 | CarD_TRCF. | |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR004576 | Mfd. | |
| InterPro | IPR005118 | TRCF. | |
| Pfam | PF02559 | CarD_TRCF | 1. |
| Pfam | PF00270 | DEAD | 1. |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF03461 | TRCF | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00580 | mfd | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=96051385; PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus
RT subtilis chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
Feature:
CHAIN 1 1177 Transcription-repair coupling factor.
/FTId=PRO_0000102162.
NP_BIND 651 658 ATP (Potential).
MOTIF 752 755 DEEQ box.
Comments:
-!- FUNCTION: Necessary for strand-specific repair. A lesion in the
template strand blocks the RNA polymerase complex (RNAP). The
RNAP-DNA-RNA complex is specifically recognized by TRCF which
releases RNAP and the truncated transcript; the TCRF may replace
RNAP at the lesion site and then recruit the uvrA/B/C repair
system (By similarity).
-!- SIMILARITY: In the N-terminal section; belongs to the uvrB family.
-!- SIMILARITY: In the C-terminal section; belongs to the helicase
family. RecG subfamily.
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Sequence length: 1177
MDNIQTFIKE SDDFKSIING LHEGLKEQLL AGLSGSARSV FTSALANETN KPIFLITHNL
YQAQKVTDDL TSLLEDRSVL LYPVNELISS EIAVASPELR AQRLDVINRL TNGEAPIVVA
PVAAIRRMLP PVEVWKSSQM LIQVGHDIEP DQLASRLVEV GYERSDMVSA PGEFSIRGGI
IDIYPLTSEN PVRIELFDTE VDSIRSFNSD DQRSIETLTS INIGPAKELI IRPEEKARAM
EKIDSGLAAS LKKLKADKQK EILHANISHD KERLSEGQTD QELVKYLSYF YEKPASLLDY
TPDNTLLILD EVSRIHEMEE QLQKEEAEFI TNLLEEGKIL HDIRLSFSFQ KIVAEQKRPL
LYYSLFLRHV HHTSPQNIVN VSGRQMQSFH GQMNVLAGEM ERFKKSNFTV VFLGANKERT
QKLSSVLADY DIEAAMTDSK KALVQGQVYI MEGELQSGFE LPLMKLAVIT EEELFKNRVK
KKPRKQKLTN AERIKSYSEL QIGDYVVHIN HGIGKYLGIE TLEINGIHKD YLNIHYQGSD
KLYVPVEQID QVQKYVGSEG KEPKLYKLGG SEWKRVKKKV ETSVQDIADD LIKLYAEREA
SKGYAFSPDH EMQREFESAF PYQETEDQLR SIHEIKKDME RERPMDRLLC GDVGYGKTEV
AIRAAFKAIG DGKQVALLVP TTILAQQHYE TIKERFQDYP INIGLLSRFR TRKEANETIK
GLKNGTVDIV IGTHRLLSKD VVYKDLGLLI IDEEQRFGVT HKEKIKQIKA NVDVLTLTAT
PIPRTLHMSM LGVRDLSVIE TPPENRFPVQ TYVVEYNGAL VREAIERELA RGGQVYFLYN
RVEDIERKAD EISMLVPDAK VAYAHGKMTE NELETVMLSF LEGESDVLVS TTIIETGVDI
PNVNTLIVFD ADKMGLSQLY QLRGRVGRSN RVAYAYFTYR RDKVLTEVAE KRLQAIKEFT
ELGSGFKIAM RDLTIRGAGN LLGAQQHGFI DSVGFDLYSQ MLKEAIEERK GDTAKTEQFE
TEIDVELDAY IPETYIQDGK QKIDMYKRFR SVATIEEKNE LQDEMIDRFG NYPKEVEYLF
TVAEMKVYAR QERVELIKQD KDAVRLTISE EASAEIDGQK LFELGNQYGR QIGLGMEGKK
LKISIQTKGR SADEWLDTVL GMLKGLKDVK KQTISST