|
|
|
|
|
|
Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 18752
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
01-OCT-1989, sequence version 1.
07-MAR-2006, entry version 54.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_BACSU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X15659 | CAA33694.1 | - |
| EMBL | Z99111 | CAB13227.1 | - |
| PIR | S04877 | XUBSM1. | |
| HSSP | P06134 | 1SFE | |
| GenomeReviews | AL009126_GR | BSU13540.1 | |
| SubtiList | BG10848 | ogt. | |
| BioCyc | BSUB1423:BSU1356-MONOMER | -.1 | |
| InterPro | IPR008332 | MethylG_mtfrase. | |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| Pfam | PF02870 | Methyltransf_1N | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=89364938; PubMed=2505068; DOI=10.1016/0921-8777(89)90022-0;
RA Kodama K., Nakabeppu Y., Sekiguchi M.;
RT "Cloning and expression of the Bacillus subtilis methyltransferase
RT gene in Escherichia coli ada- cells.";
RL Mutat. Res. 218:153-163(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=89385980; PubMed=2506524;
RA Morohoshi F., Hayashi K., Munakata N.;
RT "Bacillus subtilis gene coding for constitutive O6-methylguanine-DNA
RT alkyltransferase.";
RL Nucleic Acids Res. 17:6531-6543(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
Feature:
CHAIN 1 165 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139364.
ACT_SITE 130 130 Alkyl group acceptor (Potential).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated.
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- MISCELLANEOUS: Bacillus subtilis contains 2 species of O-6-
methylguanine-dna alkyltransferases. DAT1 and DAT2 are regulated
in different ways, constitutively and inducibly on adaptive
treatment, respectively.
-!- SIMILARITY: Belongs to the MGMT family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 165
MNYYTTAETP LGELIIAEEE DRITRLFLSQ EDWVDWKETV QNTEHKETPN LAEAKQQLQE
YFAGERKTFS LPLSQKGTPF QQKVWQALER IPYGESRSYA DIAAAVGSPK AVRAVGQANK
RNDLPIFVPC HRVIGKNSAL TGYAGSKTEI KAFLLNIERI SYKEK