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Description:
G-T mismatches repair enzyme (EC 3.2.2.-) (Thymine-DNA glycosylase)(Mismatch glycosylase) (MIG).
Molecular weight: 25401
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-APR-1993, integrated into UniProtKB/Swiss-Prot.
01-APR-1993, sequence version 1.
07-FEB-2006, entry version 48.
Phylogenetic order:
Archaea Euryarchaeota Methanobacteria Methanobacteriales Methanobacteriaceae Methanothermobacter.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein GTMR_METTF:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X68366 | CAA48433.1 | - |
| PIR | S30312 | S30312. | |
| PDB | 1KEA | X-ray | A=1-221. |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR004035 | EndoIII_FCL. | |
| InterPro | IPR004036 | EndoIII_HhH. | |
| InterPro | IPR003651 | FeS_bind. | |
| InterPro | IPR000445 | HhH. | |
| Pfam | PF00633 | HHH | 1. |
| Pfam | PF00730 | HhH-GPD | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| SMART | SM00525 | FES | 1. |
| PROSITE | PS00764 | ENDONUCLEASE_III_1 | 1. |
| PROSITE | PS01155 | ENDONUCLEASE_III_2 | 1. |
Keywords:
3D-structure; 4Fe-4S; DNA damage; DNA repair; DNA-binding; Glycosidase; Hydrolase; Hypothetical protein; Iron; Iron-sulfur; Metal-binding; Plasmid.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 3848 / THF;
RX MEDLINE=93126090; PubMed=1336177;
RA Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.;
RT "Modular organization of related Archaeal plasmids encoding different
RT restriction-modification systems in Methanobacterium
RT thermoformicicum.";
RL Nucleic Acids Res. 20:6501-6507(1992).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), AND MUTAGENESIS OF GLU-42;
RP ARG-47; TYR-126; ASP-144 AND ASN-146.
RX MEDLINE=21646524; PubMed=11786018; DOI=10.1006/jmbi.2001.5264;
RA Mol C.D., Arvai A.S., Begley T.J., Cunningham R.P., Tainer J.A.;
RT "Structure and activity of a thermostable thymine-DNA glycosylase:
RT evidence for base twisting to remove mismatched normal DNA bases.";
RL J. Mol. Biol. 315:373-384(2002).
Feature:
CHAIN 1 221 G-T mismatches repair enzyme.
/FTId=PRO_0000102237.
DNA_BIND 120 126 HHH motif.
METAL 197 197 Iron-sulfur (4Fe-4S).
METAL 204 204 Iron-sulfur (4Fe-4S).
METAL 207 207 Iron-sulfur (4Fe-4S).
METAL 213 213 Iron-sulfur (4Fe-4S).
MUTAGEN 42 42 E->D: 5-fold lower activity.
MUTAGEN 42 42 E->S: 25-fold lower activity.
MUTAGEN 47 47 R->A: 15-fold lower activity.
MUTAGEN 126 126 Y->F: 60-fold lower activity.
MUTAGEN 126 126 Y->K: No activity.
MUTAGEN 126 126 Y->S: Almost no activity.
MUTAGEN 144 144 D->A: 15-fold lower actiVITY.
MUTAGEN 144 144 D->N: No activity.
MUTAGEN 146 146 N->E,H: Almost no activity.
TURN 4 4
HELIX 5 22
STRAND 23 23
HELIX 28 31
HELIX 35 44
TURN 45 47
STRAND 48 48
HELIX 50 63
STRAND 66 66
HELIX 67 72
STRAND 73 73
HELIX 75 81
TURN 82 82
HELIX 83 85
STRAND 86 86
HELIX 88 106
TURN 107 108
STRAND 109 109
STRAND 112 112
HELIX 114 118
TURN 119 119
STRAND 120 120
TURN 121 122
HELIX 125 134
TURN 135 136
HELIX 145 155
HELIX 157 159
TURN 160 161
TURN 164 165
STRAND 166 166
HELIX 167 176
STRAND 177 177
TURN 179 180
HELIX 182 195
TURN 196 196
STRAND 197 197
STRAND 199 201
HELIX 204 206
TURN 208 212
HELIX 214 217
TURN 218 218
Comments:
-!- FUNCTION: Could act in DNA repair. Restriction methylase M.mthtI,
which is encoded by this plasmid, generates 5-methylcytosine which
is, especially under thermophilic conditions, subject to
deamination resulting in G-T mismatches. This protein could
correct these mismatches.
-!- COFACTOR: Binds 1 4Fe-4S cluster which is not important for the
catalytic activity, but which is probably involved in the proper
positioning of the enzyme along the DNA strand (By similarity).
-!- SIMILARITY: Belongs to the nth/mutY family.
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Sequence length: 221
MDDATNKKRK VFVSTILTFW NTDRRDFPWR HTRDPYVILI TEILLRRTTA GHVKKIYDKF
FVKYKCFEDI LKTPKSEIAK DIKEIGLSNQ RAEQLKELAR VVINDYGGRV PRNRKAILDL
PGVGKYTCAA VMCLAFGKKA AMVDANFVRV INRYFGGSYE NLNYNHKALW ELAETLVPGG
KCRDFNLGLM DFSAIICAPR KPKCEKCGMS KLCSYYEKCS T