Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 23066
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-OCT-2001, sequence version 1.
07-MAR-2006, entry version 29.
Phylogenetic order:
Bacteria Firmicutes Clostridia Clostridiales Clostridiaceae Clostridium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_CLOAB:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE007692 | AAK79796.1 | - |
| PIR | A97126 | A97126. | |
| HSSP | P03033 | 1JHC | |
| GenomeReviews | AE001437_GR | CAC1832.1 | |
| BioCyc | CACE1488:CAC1832-MONOMER | -.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 824 / DSM 792 / VKM B-1787;
RX MEDLINE=21359325; PubMed=11466286;
RX DOI=10.1128/JB.183.16.4823-4838.2001;
RA Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q.,
RA Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I.,
RA Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P.,
RA Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.;
RT "Genome sequence and comparative analysis of the solvent-producing
RT bacterium Clostridium acetobutylicum.";
RL J. Bacteriol. 183:4823-4838(2001).
Feature:
CHAIN 1 204 LexA repressor.
/FTId=PRO_0000170024.
DNA_BIND 31 51 H-T-H motif (By similarity).
ACT_SITE 128 128 Involved in auto-cleavage (By
similarity).
ACT_SITE 165 165 Involved in auto-cleavage (By
similarity).
SITE 92 93 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 204
MATDKITRDV QSEIYEFIRQ EVLDKGYPPS VREICAKVGL SSTSTVHGHL SRLEKKGLIR
RDPTKPRAIE LIKDPISKRE MIDIPIVGKV QAGQPILAVE NIDDYLTIPL NFVRNTNDLF
ILKISGNSMI EAGIYDGDLA IIEKTNYAQN GDIVVALIEN DATIKRFFKE KDKIRLQPEN
HTMDPIIVDN CEVIGKLAGI YRRY