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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 75523
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
31-JAN-2002, sequence version 2.
07-MAR-2006, entry version 44.
Phylogenetic order:
Bacteria Firmicutes Clostridia Clostridiales Clostridiaceae Clostridium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_CLOPE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000016 | BAB80009.1 | - |
| EMBL | X86531 | CAA60243.1 | - |
| HSSP | P56981 | 1D9Z | |
| GenomeReviews | BA000016_GR | CPE0303.1 | |
| BioCyc | CPER1502:CPE0303-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=13 / Type A;
RX MEDLINE=21664373; PubMed=11792842; DOI=10.1073/pnas.022493799;
RA Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A.,
RA Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT "Complete genome sequence of Clostridium perfringens, an anaerobic
RT flesh-eater.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 69-155.
RC STRAIN=CPN50;
RX MEDLINE=96032407; PubMed=7559358;
RA Katayama S., Dupuy B., Garnier T., Cole S.T.;
RT "Rapid expansion of the physical and genetic map of the chromosome of
RT Clostridium perfringens CPN50.";
RL J. Bacteriol. 177:5680-5685(1995).
Feature:
CHAIN 1 659 UvrABC system protein B.
/FTId=PRO_0000138389.
DOMAIN 622 657 UVR.
NP_BIND 38 45 ATP (Potential).
MOTIF 91 114 Beta-hairpin.
CONFLICT 69 75 AAQLCAE -> GSALIVK (in Ref. 2).
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 659
MGEFKIQSKF KPTGDQPKAI DTLVQSIENG NRGQTLLGVT GSGKTFTMAN IIERTQKPTL
ILAHNKTLAA QLCAEFKEFF PDNIVEYFVS YYDYYQPEAY VPQTDTFIEK DASINDEIDK
LRHSATSALL ERRDVIIVAS VSCIYGLGNP EEYKKLTISL RPGMIKDRDE VIKKLIEIQY
ERNDIDFARG TFRVRGDNLD IIPSSSSSKG IRIEFFGDEI DRIREFDVLT GNIIGERQHV
SITPASHFAA SEETLEKSIR VIEDELEDRL KVLTAEDKIL EAQRLKQRTN YDIEMIREMG
YCQGIENYSR ILDGRMPGTP PQTLLDYFPE DFLMFIDESH VTLPQVRAMY AGDRSRKTSL
VEFGFRLPCA FDNRPLKFSE FESKINQVVF VSATPGEYEL DHSEIVAEQI IRPTGLLDPV
IEIRPIQGQI DDLYGEIQRT VQRGFRVLIT TLTKRMAEDL TKYLKDLNVK ATYMHSDIDT
LERMKIIREL RLGEVDVLIG INLLREGLDI PEVALVAILD ADKEGFLRSE TSLIQTIGRA
ARNSESKVIM YADNITKSMD KSIKETERRR VIQMEYNEEH NITPTTVIKG VRDIIEATKV
SEEKENYESE VKKAAKKDIP VEKLIEQYEE EMKEAAKNLQ FERAAELRDI IKDLKENSK