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Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 24283
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
01-MAR-2004, sequence version 2.
07-MAR-2006, entry version 17.
Phylogenetic order:
Bacteria Actinobacteria Actinobacteridae Actinomycetales Corynebacterineae Corynebacteriaceae Corynebacterium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_COREF:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000035 | BAC18585.1 | ALT_INIT |
| HSSP | P24239 | 1HJR | |
| GenomeReviews | BA000035_GR | CE1775.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| TIGRFAMs | TIGR00228 | ruvC | 1. |
| PROSITE | PS01321 | RUVC | FALSE_NEG. |
Keywords:
Complete proteome; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YS-314 / AJ 12310 / DSM 44549 / JCM 11189;
RX MEDLINE=22723752; PubMed=12840036; DOI=10.1101/gr.1285603;
RA Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E.,
RA Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K.,
RA Gojobori T.;
RT "Comparative complete genome sequence analysis of the amino acid
RT replacements responsible for the thermostability of Corynebacterium
RT efficiens.";
RL Genome Res. 13:1572-1579(2003).
Feature:
CHAIN 1 223 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183092.
METAL 12 12 Magnesium (By similarity).
METAL 73 73 Magnesium (By similarity).
METAL 146 146 Magnesium (By similarity).
METAL 149 149 Magnesium (By similarity).
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group
(By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
-!- SIMILARITY: Belongs to the ruvC family.
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Sequence length: 223
MNHEGLRVMG IDPGLTRCGL SVVQAGRGRT VYPVSVGVVR TPSDAELADR LLRLSQAVGE
WMDDYTPDVI AIERVFERGN VSTVMNTAHA VGVLILAAAE RGLPVHMYTP SEVKKAISGN
GRADKKQMTV MITRILGLAE APKPADAADA LALAVCHCWR APLLMRTRMT AVDLEHRRRQ
YQGKLGKAKS TLNARNNAQV TGDAQVRAGH PSQFERPDRA DPR