Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 33009
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 21.
Phylogenetic order:
Bacteria Firmicutes Lactobacillales Lactobacillaceae Lactobacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_LACPL:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL935257 | CAD64359.1 | - |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | AL935263_GR | lp_1976.1 | |
| BioCyc | LPLA220668:LP_1976-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | FALSE_NEG. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCIMB 8826 / WCFS1;
RX MEDLINE=22480296; PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D.,
RA Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M.,
RA Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A.,
RA Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B.,
RA De Vos W.M., Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
Feature:
CHAIN 1 298 Probable endonuclease IV.
/FTId=PRO_0000190845.
METAL 69 69 Zinc 1 (By similarity).
METAL 110 110 Zinc 1 (By similarity).
METAL 145 145 Zinc 1 (By similarity).
METAL 145 145 Zinc 2 (By similarity).
METAL 179 179 Zinc 2 (By similarity).
METAL 182 182 Zinc 3 (By similarity).
METAL 214 214 Zinc 2 (By similarity).
METAL 227 227 Zinc 3 (By similarity).
METAL 229 229 Zinc 3 (By similarity).
METAL 259 259 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 298
MLRLGSHVSM KAPDMLLGSA NEAASYGANT FMIYTGAPQN TRRKPIDELK IDEAQPIIEA
HDLRQIVVHA PYIINLGNTK KPGYFEFATD FLYQEIQRAD AVGATQLTLH PGAHIGAGAN
VAITQIIKGL NAVIRPEQHV QIALETMAGK GTEVGRTFEE LAQMIDGVTY NEKLSVTFDT
CHTSDAGYAI KDDFDGVLNE FDHVIGLDRL KVIHLNDSKN PQGAHKDRHT NIGMGTIGFD
TLNKVAHHPQ LPDISKILET PYVGEDKKHQ LPPYKYEIAM LRAGQFNPHL IEDIEQQR