|
|
|
|
|
|
Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 39860
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 24.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_PSEPK:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE015451 | AAN66827.1 | - |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | AE015451_GR | PP1203.1 | |
| TIGR | PP1203 | -. | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=22423060; PubMed=12534463;
RX DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M.,
RA Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K.,
RA Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D.,
RA Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S.,
RA Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B.,
RA Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the
RT metabolically versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
Feature:
CHAIN 1 354 DNA polymerase IV.
/FTId=PRO_0000173933.
DOMAIN 6 187 UmuC.
ACT_SITE 106 106 By similarity.
METAL 10 10 Magnesium (By similarity).
METAL 105 105 Magnesium (By similarity).
SITE 15 15 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 354
MSLRKIIHVD CDCFYAAIEM RDDPRLAGRP MAVGGSPDHR GVIATCNYEA RAYGVRSAMS
SRHALKLCPD LLIVKPRFEA YREASREIHT IFRDYTELIE PLSLDEAYLD VSDSQWYSGS
ATRIAEDIRR RVARTLHITV SAGVAPNKFL AKIASDWRKP NGLFVITPNE VETFVAALPV
ARLHGVGKVT ADKLTRLGIE TCLHLREWSR LALVREFGSF GERLWGLARG IDERAVHNDS
RRQSVSVENT YDTDLPDLAS CLARLPELLD SLNERIARMD SSYRPDKPFV KVKFHDFSQT
TMEQAGAGRD LESYRQLLGQ AFARGGKPVR LLGVGVRLRD LRGAHEQLEL FPPK