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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 76190
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
01-NOV-1998, sequence version 1.
07-MAR-2006, entry version 40.
Phylogenetic order:
Bacteria Spirochaetes Spirochaetales Spirochaetaceae Treponema.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_TREPA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000520 | AAC65106.1 | - |
| PIR | C71365 | C71365. | |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | AE000520_GR | TP0116.1 | |
| TIGR | TP0116 | -. | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nichols;
RX MEDLINE=98332770; PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M.,
RA Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D.,
RA Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M.,
RA Weidman J.F., Smith H.O., Venter J.C.;
RT "Complete genome sequence of Treponema pallidum, the syphilis
RT spirochete.";
RL Science 281:375-388(1998).
Feature:
CHAIN 1 668 UvrABC system protein B.
/FTId=PRO_0000138442.
DOMAIN 627 662 UVR.
NP_BIND 38 45 ATP (Potential).
MOTIF 91 114 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 668
MKEFKLHASF QPAGDQIAAI DALVRGLHAG ARFQTLKGVT GSGKTFTVAN VIARVQKPTL
VISHNKTLSA QLYREFKGFF PDNAVEYFVS YYDYYQPESY VPARDLYIEK DASINAEINR
MRLSATFSLM ERRDVIVVAT VSCIYGLGLP ESWRDLRIHV EVNQCLDLED LKRQLVSLQY
ERNDAVLECG RFRVRGDVIE IFPAYLEEFY RIECDWDRVV RIRRIHPVSG AVLREFEELT
VYPAKHFVLK EDAIPRAMDR IRQELDERLV QLTQENKLAE AARLKTRTEY DLEMLGEMGY
CHGIENYSAP IAGRKSGEPP VTLLHYFPKD FVLFVDESHV TLPQLGAMYE GDRVRKQNLI
DFGFRLPCAR DNRPLKDSEF EALLNQAVFI SATPGVKERT QSVQIVEQLI RPTGLLDPCI
EVRKTDGQIE DICQRVKACS ARNERSLVLT LTKKMAEDLT DYFNGLGIRT KYVHSEIETI
ERVEILTSLR AGECEVLVGI NLLREGIDLP EVAFIAILDA NIVGFLRSTT SLIQIIGRAA
RNARGTVVMY ADAISDAMRE AIEETARRRK IQMAYNRAHG ITPRTIKKSI EDILVREQEV
KKDAARVQVA PLLRAADADV RTHAARKKMV QALRLHMKVC ARELRFEEAA LIRDKILQLQ
RQDEQNGV