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Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 40607
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
01-DEC-2001, sequence version 1.
07-MAR-2006, entry version 29.
Phylogenetic order:
Bacteria Firmicutes Bacillales Listeriaceae Listeria.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_LISIN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL596170 | CAC97312.1 | - |
| PIR | AH1692 | AH1692. | |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | AL592022_GR | lin2082.1 | |
| ListiList | LIN02082 | -.1 | |
| BioCyc | LINN1642:LIN2082-MONOMER | -.1 | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLIP 11262 / Serovar 6a;
RX MEDLINE=21537279; PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A.,
RA Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T.,
RA Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P.,
RA Domann E., Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O.,
RA Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P.,
RA Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D.,
RA Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G.,
RA Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H.,
RA Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R.,
RA Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A.,
RA Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
Feature:
CHAIN 1 356 DNA polymerase IV.
/FTId=PRO_0000173921.
DOMAIN 1 188 UmuC.
ACT_SITE 107 107 By similarity.
METAL 11 11 Magnesium (By similarity).
METAL 106 106 Magnesium (By similarity).
SITE 16 16 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 356
MDTSRKIIHI DMDAFYASVE QRDHPEFRGK PLIIGGDPNK RGVVATCSYE ARKFGVHSAM
PTRQAAKLCP NGIFIHGNMA HYVEVSNQIR EIFSRYTDII EPLSLDEAYL DVTENKKGMK
SATMVAREIQ QTIYRELGLT ASAGVSFNKF IAKIASDFKK PAGITVVAPE EAEAFLEQIP
VTKFYGVGKV TAEKLHRLGI ETGADLKKWS EWDLIRELHK HGYQLYRHVR GRSNNIVNPH
RDRKSVGKET TFEFNVLDNR ILEQSLMKFA KKVEERLIKL QKHGKTVVLK LRYSDFTTIT
KRLTLNEYTN DANQIYQAAA LLLRESYKGQ DSIRLIGLTV TNLKPVYFEN LRLEGL