Description:
Exodeoxyribonuclease (EC 3.1.11.2).
Molecular weight: 31064
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
15-MAR-2005, sequence version 1.
07-MAR-2006, entry version 9.
Phylogenetic order:
Bacteria Firmicutes Lactobacillales Streptococcaceae Streptococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EXOA_STRR6:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | J04234 | AAA26879.1 | - |
| EMBL | AE008531 | AAL00463.1 | - |
| PIR | A32301 | A32301. | |
| PIR | B98079 | B98079. | |
| HSSP | P27695 | 1E9N | |
| GenomeReviews | AE007317_GR | spr1660.1 | |
| InterPro | IPR000097 | APendonclse1. | |
| InterPro | IPR005135 | Exo_endo_phos. | |
| InterPro | IPR004808 | ExoIII_xth. | |
| Pfam | PF03372 | Exo_endo_phos | 1. |
| TIGRFAMs | TIGR00195 | exoDNase_III | 1. |
| TIGRFAMs | TIGR00633 | xth | 1. |
| PROSITE | PS00726 | AP_NUCLEASE_F1_1 | 1. |
| PROSITE | PS00727 | AP_NUCLEASE_F1_2 | 1. |
| PROSITE | PS00728 | AP_NUCLEASE_F1_3 | 1. |
Keywords:
Complete proteome; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=89213905; PubMed=2468646;
RA Puyet A., Greenberg B., Lacks S.A.;
RT "The exoA gene of Streptococcus pneumoniae and its product, a DNA
RT exonuclease with apurinic endonuclease activity.";
RL J. Bacteriol. 171:2278-2286(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=21429245; PubMed=11544234;
RX DOI=10.1128/JB.183.19.5709-5717.2001;
RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E.,
RA LeBlanc D.J., Lee L.N., Lefkowitz E.J., Lu J., Matsushima P.,
RA McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I.,
RA Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P.,
RA Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G.,
RA Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L.,
RA Glass J.I.;
RT "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL J. Bacteriol. 183:5709-5717(2001).
Feature:
CHAIN 1 275 Exodeoxyribonuclease.
/FTId=PRO_0000200027.
ACT_SITE 266 266 Proton acceptor (By similarity).
METAL 46 46 Magnesium or manganese (By similarity).
Comments:
-!- FUNCTION: In addition to 3' to 5' exonuclease and 3'-phosphatase
activities, exoA was shown to make single-strand breaks at
apurinic sites in DNA.
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage in the 3'- to 5'-
direction to yield nucleoside 5'-phosphates.
-!- SUBCELLULAR LOCATION: Cytoplasm.
-!- SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family.
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Sequence length: 275
MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK GPTKKHVEIL
EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP EIGAPSTMDL EGRIITLEFD
AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA EYLAELDKEK PVLATGDYNV AHNEIDLANP
ASNRRSPGFT DEERAGFTNL LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID
YWLTSNRIAD KVTKSDMIDS GARQDHTPIV LEIDL