Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 50688
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
19-OCT-2002, sequence version 1.
07-MAR-2006, entry version 26.
Phylogenetic order:
Bacteria Actinobacteria Actinobacteridae Actinomycetales Corynebacterineae Corynebacteriaceae Corynebacterium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_CORGL:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000036 | BAB99537.1 | - |
| EMBL | BX927154 | CAF20484.1 | - |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | BX927147_GR | cg2355.1 | |
| GenomeReviews | BA000036_GR | Cgl2144.1 | |
| BioCyc | CGLU196627:NCGL2064-MONOMER | -.1 | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / NCIB 10025;
RA Nakagawa S.;
RT "Complete genomic sequence of Corynebacterium glutamicum ATCC 13032.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / NCIB 10025;
RX MEDLINE=22830012; PubMed=12948626; DOI=10.1016/S0168-1656(03)00154-8;
RA Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M.,
RA Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L.,
RA Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B.,
RA McHardy A.C., Meyer F., Moeckel B., Pfefferle W., Puehler A.,
RA Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I.,
RA Tauch A.;
RT "The complete Corynebacterium glutamicum ATCC 13032 genome sequence
RT and its impact on the production of L-aspartate-derived amino acids
RT and vitamins.";
RL J. Biotechnol. 104:5-25(2003).
Feature:
CHAIN 1 467 DNA polymerase IV.
/FTId=PRO_0000173911.
DOMAIN 5 187 UmuC.
ACT_SITE 105 105 By similarity.
METAL 9 9 Magnesium (By similarity).
METAL 104 104 Magnesium (By similarity).
SITE 14 14 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 467
MQRWVLHIDM DAFFASCEQL TRPTLRGRPV LVGGVSGRGV VAGASYEARK FGARSAMPMH
QAKARVGFGA VVVTPRHIVY SAASRRVFQI VEKRAGIVER LSIDEGFMEP EALVGATPEE
VKQWAEELRA EIKEVTGLPS SVGAGSGKQI AKIGSGEAKP DGVFVVPVDK QHDLLDPLPV
GALWGVGPVT GSKLASMGVE TIGDLAALTQ KEVEISLGAT IGISLWNLAR GIDDRPVEPR
AEAKQISQEH TYEKDLLTRQ QVDAAIIRSA EGAHRRLLKD GRGARTVSVK LRMADFRIES
RSYTLSYATD DYATLEATAF RLARYPGEVG PIRLVGVSFS GLEESRQDIL FPELDQQIIV
PPAPDTDYEV GVQSSSSSES TQVEAPQDVA LSMWCATQDV YHPEYGHGWV QGAGHGVVSV
RFETRSTTKG RTKSFSMDDP DLTPADPLDS LDWADWFAEN GETGDDE