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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 75955
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
01-MAR-2003, sequence version 1.
07-MAR-2006, entry version 19.
Phylogenetic order:
Bacteria Spirochaetes Spirochaetales Leptospiraceae Leptospira.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_LEPIN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE011251 | AAN47848.1 | - |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | AE010300_GR | LA0649.1 | |
| BioCyc | LINT189518:LA0649-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=56601 / Serogroup Icterohaemorrhagiae / Serovar lai;
RX MEDLINE=22598143; PubMed=12712204; DOI=10.1038/nature01597;
RA Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H.,
RA Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F.,
RA Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H., Yin H.-F.,
RA Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W.,
RA Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A.,
RA Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z.,
RA Xu J.-G., Zhao G.-P.;
RT "Unique physiological and pathogenic features of Leptospira
RT interrogans revealed by whole-genome sequencing.";
RL Nature 422:888-893(2003).
Feature:
CHAIN 1 666 UvrABC system protein B.
/FTId=PRO_0000138402.
DOMAIN 625 660 UVR.
NP_BIND 39 46 ATP (Potential).
MOTIF 92 115 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 666
MASVFKIHSA YQPAGDQVKA IQNIADSFQK GEKKVTLVGV TGSGKTFTMA QVIQNLGLPT
LVLSHNKTLA AQLFREFKEF FPENAVEYFV SYYDYYQPEA YVPSSDTFIE KDSSINEEID
KLRLRATSSL LEREDVVIVS SVSCIYGLGS PEEYTNSVVA LKVGDTIERD TVIRKLLHIQ
YNRNDLDFSR GNFRVRGDSI EIYPAYHTDG IRIEFFGDEI DSISRINPVT AQTIFKLEKA
YIYPAKHFIT SGPKVKEAVE NIRAEVDAQT DFFRKNNKLL EAERILSRTN YDMEMLQEMG
YCNGIENYSR HLTGRKPGER PACLIDYFQG EFLLIVDESH VTIPQIGGMF AGDRARKQTL
VDFGFRLPSA LDNRPLNFQE FETLTPRTLY VSATPAEYEI EKSSKVVEQI IRPTGLLDPI
VDVRPTKNQI EDLLVEIRKR IDAGERVLVT TLTKKMSEDL TDYYEEIGLK VAYLHSEVET
LDRVGIIRDL RKGIYDVLIG INLLREGLDI PEVSLVAILD ADKEGFLRNY KSLIQTIGRA
ARNVNGTAIL YADKTTDSMA KAIEETKRRR KIQEDHNLKF GITPLTIKKE VGDIIEREEK
ERTSEDLVLE DVEKKFNSKK FPNKEVLKEK LREEMMKAAK ELDFERAAIL RDKMLSIQTE
DSSAKN