Description:
Flap structure-specific endonuclease (EC 3.1.-.-).
Molecular weight: 37170
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
07-MAR-2006, entry version 37.
Phylogenetic order:
Archaea Euryarchaeota Methanobacteria Methanobacteriales Methanobacteriaceae Methanothermobacter.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein FEN_METTH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000666 | AAB86106.1 | - |
| PIR | C69085 | C69085. | |
| HSSP | O93634 | 1B43 | |
| GenomeReviews | AE000666_GR | MTH1633.1 | |
| BioCyc | MTHE187420:MTH1633-MONOMER | -.1 | |
| LinkHub | O27670 | -.1 | |
| HAMAP | MF_00614 | - | 1. |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR006086 | XPG_I. | |
| InterPro | IPR006085 | XPG_N. | |
| InterPro | IPR006084 | XPGC_Rad. | |
| Pfam | PF00867 | XPG_I | 1. |
| Pfam | PF00752 | XPG_N | 1. |
| PRINTS | PR00853 | XPGRADSUPER. | |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00484 | XPGI | 1. |
| SMART | SM00485 | XPGN | 1. |
| PROSITE | PS00841 | XPG_1 | 1. |
Keywords:
Complete proteome; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Delta H;
RX MEDLINE=98037514; PubMed=9371463;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum
RT deltaH: functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
Feature:
CHAIN 1 328 Flap structure-specific endonuclease.
/FTId=PRO_0000154056.
METAL 154 154 Magnesium 1 (By similarity).
Comments:
-!- FUNCTION: Endonuclease that cleave the 5'overhanging flap
structure that is generated by displacement synthesis when DNA
polymerase encounters the 5'end of a downstream Okazaki fragment.
Has 5'endo-/exonuclease and 5'pseudo-Y-endonuclease activities.
Cleaves the junction between single and double-stranded regions of
flap DNA (By similarity).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
subfamily.
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Sequence length: 328
MGVKLRDVVS PRRIRLEDLR GRTVAVDAAN TLYQFLSSIR QRDGTPLMDS RGRVTSHLSG
ILYRTAAVME REIRVIYVFD GRSHHLKGET VSRRADIRKK SEVEWKRALE EGDIDRAKKY
AVRSSRMSSE ILESSKRLLE LLGIPYVQAP GEGEAQASYM VKMGDAWAVA SQDYDCLLFG
APRVVRNLTL SGKLEDPEII ELESTLRELS ISHTQLVDMA LLVGTDFNEG VKGIGARRGL
KLIREKGDIF KVIRDLEADI GGDPQVLRRI FLEPEVSEDY EIRWRKPDVE GVIEFLCTEH
GFSEDRVRAA LKKFEGASST QKSLEDWF