Description:
Probable N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNAglycosylase (EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase(EC 4.2.99.18) (AP lyase)].
Molecular weight: 36052
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
07-MAR-2006, entry version 43.
Phylogenetic order:
Archaea Euryarchaeota Methanobacteria Methanobacteriales Methanobacteriaceae Methanothermobacter.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGG1_METTH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000666 | AAB85820.1 | - |
| PIR | E69045 | E69045. | |
| HSSP | Q9UIK1 | 1LWY | |
| GenomeReviews | AE000666_GR | MTH1342.1 | |
| BioCyc | MTHE187420:MTH1342-MONOMER | -.1 | |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR004577 | Ogg. | |
| InterPro | IPR012904 | OGG_N. | |
| Pfam | PF00633 | HHH | 1. |
| Pfam | PF00730 | HhH-GPD | 1. |
| Pfam | PF07934 | OGG_N | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| TIGRFAMs | TIGR00588 | ogg | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase; Lyase; Multifunctional enzyme.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Delta H;
RX MEDLINE=98037514; PubMed=9371463;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum
RT deltaH: functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
Feature:
CHAIN 1 312 Probable N-glycosylase/DNA lyase.
/FTId=PRO_0000058596.
ACT_SITE 235 235 By similarity.
Comments:
-!- FUNCTION: DNA repair enzyme that incises DNA at 8-oxoG residues.
Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-
methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-
lyase activity that nicks DNA 3' to the lesion (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage near apurinic or
apyrimidinic sites to products with 5'-phosphate.
-!- SIMILARITY: Belongs to the type-1 OGG1 family.
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Sequence length: 312
MRIPVGDFDL EMTQRSGQTS QPPWREVEGA FRELLIIEGV PCPVEVRNEA GVLRVRPYVD
VPQKTLREKI EYIFDLKFDI EDFYTFLEDK NLSYTLDSSR GLRLFLAKDP FECVISSIAS
ANCSVVRWTR SIEDIRRLWG QANTFNGETF HTFPSPHVLT GVAEGSLEDL QRAEDNLPSD
FSFNDLRSCG VGYRAPYIRE TSRILAEEMD IRRIDGMDYD DARELLLELS GVGPKVADCI
LLYGFRKTEA FPVDVWIRRI MNHIHPGRNF NDRSMVEFAR REYGEMADYV QLYLFNHARR
SGLLDRLRQG TG