Description:
Deoxyribodipyrimidine photo-lyase (EC 4.1.99.3) (DNA photolyase)(Photoreactivating enzyme).
Molecular weight: 51762
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 56.
Phylogenetic order:
Archaea Euryarchaeota Methanobacteria Methanobacteriales Methanobacteriaceae Methanothermobacter.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein PHR_METTH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D30752 | BAA06411.1 | - |
| EMBL | AE000666 | AAB85402.1 | - |
| PIR | A69221 | A69221. | |
| GenomeReviews | AE000666_GR | MTH904.1 | |
| BioCyc | MTHE187420:MTH904-MONOMER | -.1 | |
| InterPro | IPR008148 | DNA_photolyase_2. | |
| InterPro | IPR006050 | DNA_photolyase_N. | |
| InterPro | IPR008149 | FAD_bd_C. | |
| InterPro | IPR006051 | FAD_bd_N. | |
| InterPro | IPR005101 | Photolyse_FAD_bd. | |
| PANTHER | PTHR10211 | DNA_photolyase_2.1 | 1. |
| Pfam | PF00875 | DNA_photolyase | 1. |
| Pfam | PF03441 | FAD_binding_7 | 1. |
| ProDom | PD007711 | FAD_binding_C | 1. |
| ProDom | PD004390 | FAD_binding_N | 1. |
| TIGRFAMs | TIGR00591 | phr2 | 1. |
| PROSITE | PS01083 | DNA_PHOTOLYASES_2_1 | 1. |
| PROSITE | PS01084 | DNA_PHOTOLYASES_2_2 | 1. |
Keywords:
Chromophore; Complete proteome; DNA damage; DNA repair; DNA-binding; FAD; Flavoprotein; Lyase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Delta H;
RX MEDLINE=95112825; PubMed=7813451;
RA Yasui A., Eker A.P., Yasuhira S., Yajima H., Kobayashi T., Takao M.,
RA Oikawa A.;
RT "A new class of DNA photolyases present in various organisms including
RT aplacental mammals.";
RL EMBO J. 13:6143-6151(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Delta H;
RX MEDLINE=98037514; PubMed=9371463;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum
RT deltaH: functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
Feature:
CHAIN 1 445 Deoxyribodipyrimidine photo-lyase.
/FTId=PRO_0000085117.
BINDING 239 239 DNA (By similarity).
Comments:
-!- FUNCTION: Involved in repair of UV radiation-induced DNA damage.
Catalyzes the light-dependent monomerization (300-600 nm) of
cyclobutyl pyrimidine dimers (in cis-syn configuration), which are
formed between adjacent bases on the same DNA strand upon exposure
to ultraviolet radiation.
-!- CATALYTIC ACTIVITY: Cyclobutadipyrimidine (in DNA) = 2 pyrimidine
residues (in DNA).
-!- COFACTOR: Binds 1 FAD per subunit.
-!- COFACTOR: Binds 1 8-hydroxy-5-deazaflavin (F420) noncovalently per
subunit.
-!- SIMILARITY: Belongs to the DNA photolyase class-2 family.
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Sequence length: 445
MIHAERIRNL NGEEPDLRGS YVVYWMQASV RSHWNHALEY AIETANSLKK PLIVVFGLTD
DFPNANSRHY RFLIEGLRDV RSNLRERGIQ LVVERDSPPS VLLKYADDAA AAVTDRGYLD
IQKEWVDEAA GALHIPLTQV ESNVIVPVET ASDKEEYSAG TFKPKIKRHL KRFMVPLRMR
TLKMDSLDLE PGPEFEDAVR DFRAPEDLEP SVFRGGTSTA LSIFSEFLRE KLECFERYRN
DPVKNCLSNM SPYLHFGQIS PLYLALRASE AGECPEFLEE LIVRRELSMN FVHYSDSYSS
ISCLPEWAQR TLMDHVADPR EYEYSLRELE SASTHDPYWN AAQQEMVITG KMHGYMRMYW
GKKILEWTDH PARAYDIALY LNDRYEIDGR DPNGFAGVAW CFGKHDRAWA EREIFGKVRY
MNDRGLKRKF RIDEYVDRIR GLMDE