Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31070
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
01-MAY-2000, sequence version 1.
07-MAR-2006, entry version 43.
Phylogenetic order:
Bacteria Actinobacteria Actinobacteridae Actinomycetales Streptomycineae Streptomycetaceae Streptomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_STRCO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL939111 | CAB51959.1 | - |
| PIR | T35492 | T35492. | |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | AL645882_GR | SCO2111.1 | |
| BioCyc | SCOE1902:SCO2111-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A3(2) / M145;
RX MEDLINE=21996410; PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H.,
RA Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M.,
RA Cronin A., Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S.,
RA Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S.,
RA Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S.,
RA Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K.,
RA Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J.,
RA Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces
RT coelicolor A3(2).";
RL Nature 417:141-147(2002).
Feature:
CHAIN 1 294 Probable endonuclease IV.
/FTId=PRO_0000190878.
METAL 78 78 Zinc 1 (By similarity).
METAL 118 118 Zinc 1 (By similarity).
METAL 157 157 Zinc 1 (By similarity).
METAL 157 157 Zinc 2 (By similarity).
METAL 191 191 Zinc 2 (By similarity).
METAL 194 194 Zinc 3 (By similarity).
METAL 228 228 Zinc 2 (By similarity).
METAL 241 241 Zinc 3 (By similarity).
METAL 243 243 Zinc 3 (By similarity).
METAL 273 273 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 294
MSTASSSSSL PRNPVGGHVP VAGGLHSVGL SYARELKAEA VQVFVANPRG WATPAGNPKQ
DEAFREACAA GSVPAYVHAP YLINFGSHTG ATVERSVESL RHSLRRGRAI GALGVVVHTG
SATGGRERPV ALKQVREHML PLLDELTHDD DPYLLLESTA GQGASLCSRT WDFGPYFEAL
DAHPKLGVCL DTCHIFAAGH DLTGPSGMHQ TLDLLVDTVG EGRLRLIHAN DSKDVAGAHK
DRHENIGAGH IGEDPFRALM THPATDGVPL VIETPGGKEG HAADVARLKK LRDG