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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 75388
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
01-FEB-1996, sequence version 1.
07-MAR-2006, entry version 46.
Phylogenetic order:
Bacteria Firmicutes Mollicutes Mycoplasmataceae Mycoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_MYCGE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L43967 | AAC71474.1 | - |
| EMBL | U02152 | AAD12433.1 | - |
| EMBL | U01761 | AAD10575.1 | - |
| EMBL | U01761 | AAD10576.1 | ALT_SEQ |
| PIR | A64228 | A64228. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | L43967_GR | MG254.1 | |
| TIGR | MG254 | -. | |
| BioCyc | MGEN2097:MG254-MONOMER | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | FALSE_NEG. |
| PROSITE | PS01055 | DNA_LIGASE_N1 | 1. |
| PROSITE | PS01056 | DNA_LIGASE_N2 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / G-37;
RX MEDLINE=96026346; PubMed=7569993;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-122 AND 477-574.
RC STRAIN=ATCC 33530 / G-37;
RX MEDLINE=94075230; PubMed=8253680;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random
RT sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
Feature:
CHAIN 1 659 DNA ligase.
/FTId=PRO_0000161749.
ACT_SITE 113 113 N6-AMP-lysine intermediate (By
similarity).
CONFLICT 62 72 VGGEAVKGFKK -> WEEKLWRVLKS (in Ref. 2).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA (By similarity).
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
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Sequence length: 659
MMDVKLKIQQ LVNLIKNYDY HYYVLSEPLI DDFEYDMLYK SLQQLEKDHP DLIQIDSPTQ
RVGGEAVKGF KKLNHNSPML SLENAFSTKE IANFIDNINF QTNSKNEFVV EPKIDGVSIS
LTYKNGVLVH ALTRGDGSVG EDVLNNVKTI KSIPLTIPFT KTIEIRGEIF VDKKTFLAIN
NQLEKPFANA RNLAAGTIRN LNSEITAQRK LRALFYYIPN GLEESITTQT MVLEQLKQWK
FPVSDTIRVF QNKFQLINYL EAFDKKREQL TFNLDGLVIK LNSLLFYQQL GATSKSPRWA
IAFKFSPKFV QTKLTAVLIT IGRTGRVNYT AKLESVNLDG TKVTAATLHN FDYIKTKDIR
INDTVVIYKA GEIIPKVLKV NLEKRKNDTI IIQEQKYCPS CNSKLVKIVD EVDQYCTNET
CKERNIQLIN YFVSKTAMDI NGLNINTITK LYEHNLVRSI VDLYDLKDKK NQVLKLDLKI
GDKLFNKLVD NIENSKQKGM ARLLTGLGIK HVGNVLAKNL ANHFKNIKAL QHASLENLIS
LNDVGITVAE SLYNWFHDPN HLQLIEQLEL RQVKTDQLPL KINFETNSIY FQKRFLITGS
FNISRDQIKD LLSAKFDCQF ASEVKPTVDF VIAGNKPTLR KINHAKELNI PIINEAIWT