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Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 32391
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
01-FEB-1996, sequence version 1.
07-MAR-2006, entry version 40.
Phylogenetic order:
Bacteria Firmicutes Mollicutes Mycoplasmataceae Mycoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_MYCGE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L43967 | AAC71456.1 | - |
| PIR | I64225 | I64225. | |
| GenomeReviews | L43967_GR | MG235.1 | |
| TIGR | MG235 | -. | |
| BioCyc | MGEN2097:MG235-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / G-37;
RX MEDLINE=96026346; PubMed=7569993;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
Feature:
CHAIN 1 291 Probable endonuclease IV.
/FTId=PRO_0000190852.
METAL 72 72 Zinc 1 (By similarity).
METAL 112 112 Zinc 1 (By similarity).
METAL 147 147 Zinc 1 (By similarity).
METAL 147 147 Zinc 2 (By similarity).
METAL 181 181 Zinc 2 (By similarity).
METAL 184 184 Zinc 3 (By similarity).
METAL 215 215 Zinc 2 (By similarity).
METAL 228 228 Zinc 3 (By similarity).
METAL 230 230 Zinc 3 (By similarity).
METAL 260 260 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 291
MPKLLGSFIS FKAPNYFVQS AQDAIAIDAT ALMVFLGPPH SAYRVPFNKM QFSLGYELLK
TKNINSNGLV VHAPYIINCA SKDPLKQQNA ISVLTNEIQL CNLAGAHYLV LHPGSAVAQT
TNEALDNLVK VLNQVINKTK TTVICLETMA GKGNEIGRDL TELKYVIDRI VDKDRIGVCL
DTCHFHDSGI DFSDLTGVFN TITTKLGFEF LKVIHLNESK NNCGSKKDRH ANINAGMIGF
ENLMKFISHP QIKDLPIILE TPSTSLNYPT IYREEISQIR SWFKTYQPDA N