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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 75202
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
01-FEB-1996, sequence version 1.
07-MAR-2006, entry version 42.
Phylogenetic order:
Bacteria Firmicutes Mollicutes Mycoplasmataceae Mycoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_MYCGE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L43967 | AAC71291.1 | - |
| EMBL | U01743 | AAD10554.1 | ALT_INIT |
| EMBL | U02119 | AAD12393.1 | - |
| EMBL | U01698 | AAB01010.1 | - |
| PIR | A64208 | A64208. | |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | L43967_GR | MG073.1 | |
| TIGR | MG073 | -. | |
| BioCyc | MGEN2097:MG073-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / G-37;
RX MEDLINE=96026346; PubMed=7569993;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-78; 102-231 AND 414-528.
RC STRAIN=ATCC 33530 / G-37;
RX MEDLINE=94075230; PubMed=8253680;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random
RT sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
Feature:
CHAIN 1 656 UvrABC system protein B.
/FTId=PRO_0000138407.
DOMAIN 614 649 UVR.
NP_BIND 42 49 ATP (Potential).
MOTIF 95 118 Beta-hairpin.
CONFLICT 45 45 G -> A (in Ref. 2).
CONFLICT 102 107 EAYLPS -> KLTYPD (in Ref. 2).
CONFLICT 432 433 DN -> IT (in Ref. 2).
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 656
MKNQTKSNSL FQLSTNYIPT GDQPEAIKKL SEFKTKQQVL LGATGTGKTF TIANVIQNSQ
LPTVVIAHNK TLAGQLFNEL KQLFPKNAVE YFISYFDFYQ PEAYLPSKGI YIEKSATVNE
AIKRLRVSTL HSLSTRKDVI VVGSVASIYP TSSPSDFVKY CLWFVVGKDY DLKTIKDRLV
SLNYVVNKQQ LTPGKFRFQG DVLEVFPGYS DAFVIRISFF DTKVEQICQI DPLTNKILNQ
LFEIKIGPAD EYVVNQSDLD IAIKNIKQEL QERVNYFNKQ NLVERAQRLA TITNHDLNDL
KAWGFCSGVE NYARHLELRM ANSTPYSIFD YFKGDWLLVI DESHQTLPQL NGMYNTDLSR
KQSLIDYGFR LPSALDNRPL SFAELQQKMQ KVIYVSATPR DKEISLSQNN VIEQLVRPTY
LVDPIIVVKP KDNQVEDLIE EIINQRQNNT RTFVTVLTIK MAENLTEYLK ERKIKVAYIH
KDIKALERLL LINDLRRGEY ECLVGINLLR EGLDVPEVAL VCIFDADIPG LPRDERSLIQ
IIGRAARNEH GRVVMYANHV TEQMQKAIDE TKRRRTVQME YNKLHNKTPK TVVKPLTFVQ
PIKLKAKSNA EKNAALIKQL TKEMKKAAAN QNYELAIEIR DSIFELEKEI GSKIKV