Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31890
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
01-FEB-1997, sequence version 1.
07-MAR-2006, entry version 39.
Phylogenetic order:
Bacteria Firmicutes Mollicutes Mycoplasmataceae Mycoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_MYCPN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U00089 | AAB96156.1 | - |
| PIR | S73834 | S73834. | |
| GenomeReviews | U00089_GR | MPN328.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29342 / M129;
RX MEDLINE=97105885; PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C.,
RA Herrmann R.;
RT "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT pneumoniae.";
RL Nucleic Acids Res. 24:4420-4449(1996).
Feature:
CHAIN 1 286 Probable endonuclease IV.
/FTId=PRO_0000190858.
METAL 72 72 Zinc 1 (By similarity).
METAL 112 112 Zinc 1 (By similarity).
METAL 147 147 Zinc 1 (By similarity).
METAL 147 147 Zinc 2 (By similarity).
METAL 181 181 Zinc 2 (By similarity).
METAL 184 184 Zinc 3 (By similarity).
METAL 215 215 Zinc 2 (By similarity).
METAL 228 228 Zinc 3 (By similarity).
METAL 230 230 Zinc 3 (By similarity).
METAL 260 260 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 286
MPKLLGSFIS FKSPHYLVGS VRDAVSIKAQ AFMIFLGAPH TALRVDPNRM QIDEGHTLME
QHNLSKSGMV VHAPYIINCA SKDPVKQTFA IDVLTREVKL CHAVGAKLIV LHPGSAVEQT
QTQALDHLIK VLNTVIANTK EVIICLETMA GKGNEIGRDL DQLKYVINHI EQQERIGVCL
DTCHFHDSGN DFNNTAEIME TIDTKLGFEF LKVIHLNESK NVCGSKKDRH ANLGEGMIGF
DNLMRFIAQP QIKQIPIVLE TPSDKHNYPA VYGAEIERIR AWFGAR